AKAP8 Gene

HGNC Family	A-kinase anchor proteins (AKAP)
Name	A kinase (PRKA) anchor protein 8
Description	This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n A‐kinase anchoring protein 8 (AKAP8), also known as AKAP95, acts as a versatile nuclear scaffold that integrates multiple aspects of gene regulation and cell cycle control. In the nucleus it directly regulates pre–mRNA splicing by binding RNA and coordinating with splice regulatory proteins – a function that contributes to maintaining epithelial cell identity and impeding the epithelial–mesenchymal transition."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In addition, AKAP8 plays critical roles in DNA replication and mitotic progression: it acts as a scaffold for MCM2 to ensure efficient origin firing"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ", and it partners with histone deacetylase 3 (HDAC3) on mitotic chromosomes to facilitate proper histone H3 deacetylation and subsequent chromatin modifications."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Moreover, AKAP8 is instrumental in modulating chromatin states by interacting with core subunits of histone H3K4 methyltransferase complexes—via its PKA‐binding domain and interaction with DPY30—to directly influence gene expression programs."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond chromatin and transcript processing, AKAP8 orchestrates nuclear cyclic AMP (cAMP) signaling by anchoring protein kinase A (PKA) and associated phosphodiesterases into specialized nuclear microdomains."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " It also serves unexpected roles in apoptosis by binding active caspase‐3 and facilitating its transport into the nucleus to mediate nuclear morphological changes"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": ", and in the nucleolus where it influences rRNA production."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " Finally, AKAP8 contributes to the maintenance of mitotic fidelity by interacting with nuclear pore components such as TPR to support the spindle assembly checkpoint"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": ", and it is implicated in tissue‐specific gene regulation such as promoting CYP19A1 expression and estrogen synthesis in ovarian granulosa cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiaohui Hu, Samuel E Harvey, Rong Zheng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The RNA-binding protein AKAP8 suppresses tumor metastasis by antagonizing EMT-associated alternative splicing."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-020-14304-1"}], "href": "https://doi.org/10.1038/s41467-020-14304-1"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31980632"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31980632"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jing Hu, Alireza Khodadadi-Jamayran, Miaowei Mao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncomms13347"}], "href": "https://doi.org/10.1038/ncomms13347"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27824034"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27824034"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Turid Eide, Kristin A Taskén, Cathrine Carlson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M300765200"}], "href": "https://doi.org/10.1074/jbc.M300765200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12740381"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12740381"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Estelle A Wall, Joelle R Zavzavadjian, Mi Sook Chang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Signal (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/scisignal.2000202"}], "href": "https://doi.org/10.1126/scisignal.2000202"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19531803"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19531803"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Anna Bieluszewska, Martyna Weglewska, Tomasz Bieluszewski, et al. "}, {"type": "b", "children": [{"type": "t", "text": "PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "FEBS J (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/febs.14378"}], "href": "https://doi.org/10.1111/febs.14378"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29288530"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29288530"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Terri Clister, Eric C Greenwald, George S Baillie, et al. "}, {"type": "b", "children": [{"type": "t", "text": "AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for Regulating Nuclear PKA."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Chem Biol (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.chembiol.2019.03.003"}], "href": "https://doi.org/10.1016/j.chembiol.2019.03.003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30982750"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30982750"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Malcolm W J MacDougall, G Nicholas Europe-Finner, Stephen C Robson "}, {"type": "b", "children": [{"type": "t", "text": "Human myometrial quiescence and activation during gestation and parturition involve dramatic changes in expression and activity of particulate type II (RII alpha) protein kinase A holoenzyme."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Endocrinol Metab (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1210/jc.2002-021862"}], "href": "https://doi.org/10.1210/jc.2002-021862"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12727975"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12727975"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Shinji Kamada, Ushio Kikkawa, Yoshihide Tsujimoto, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biol (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/MCB.25.21.9469-9477.2005"}], "href": "https://doi.org/10.1128/MCB.25.21.9469-9477.2005"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16227597"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16227597"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Anne Marstad, Ole Jørgen B Landsverk, Olaf Strømme, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A-kinase anchoring protein AKAP95 is a novel regulator of ribosomal RNA synthesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "FEBS J (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/febs.13630"}], "href": "https://doi.org/10.1111/febs.13630"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26683827"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26683827"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Graciela López-Soop, Torunn Rønningen, Agnieszka Rogala, et al. "}, {"type": "b", "children": [{"type": "t", "text": "AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Cycle (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/15384101.2017.1310350"}], "href": "https://doi.org/10.1080/15384101.2017.1310350"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28379780"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28379780"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Yu Gu, Wenbin Xu, Bole Zhuang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Role of A-kinase anchoring protein 95 in the regulation of cytochrome P450 family 19 subfamily A member 1 (CYP19A1) in human ovarian granulosa cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Reprod Fertil Dev (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1071/RD17313"}], "href": "https://doi.org/10.1071/RD17313"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29397057"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29397057"}]}]}]}
Synonyms	AKAP95, AKAP-8, AKAP-95, AKAP 95
Proteins	AKAP8_HUMAN
NCBI Gene ID	10270
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

AKAP8 has 8,907 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 125 datasets.

Click the + buttons to view associations for AKAP8 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

AKAP8 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by AKAP8 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of AKAP8 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of AKAP8 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of AKAP8 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of AKAP8 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of AKAP8 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of AKAP8 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	Biocarta Pathways	pathways involving AKAP8 protein from the Biocarta Pathways dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of AKAP8 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of AKAP8 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of AKAP8 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of AKAP8 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of AKAP8 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of AKAP8 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with AKAP8 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with AKAP8 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of AKAP8 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of AKAP8 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of AKAP8 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of AKAP8 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing AKAP8 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing AKAP8 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing AKAP8 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing AKAP8 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with AKAP8 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with AKAP8 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of AKAP8 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with AKAP8 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with AKAP8 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with AKAP8 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by AKAP8 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with AKAP8 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with AKAP8 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with AKAP8 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with AKAP8 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at AKAP8 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of AKAP8 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of AKAP8 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing AKAP8 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of AKAP8 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.