ARF5 Gene

HGNC Family	Ras small GTPases superfamily
Name	ADP-ribosylation factor 5
Description	This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n The small GTPase ARF5, a member of the class II ARF proteins, plays multifaceted roles in regulating membrane trafficking events at the Golgi apparatus and recycling endosomes, where it often functions in concert with ARF4. ARF5 contributes to the recruitment of guanine nucleotide exchange factors and coat complexes at pre‐Golgi and trans‐Golgi network (TGN) compartments, thereby participating in the formation and maintenance of vesicular carriers required for protein secretion (see"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "and."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " In addition, ARF5 interacts with specific effector proteins, including members of the arfophilin family, which bind ARF5 in a nucleotide‐dependent manner to modulate endosomal recycling and even influence cytokinetic processes through dual interactions with Rab11 and Arf6 (see"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "and."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " ARF5 is also critical in specialized vesicular trafficking events such as clathrin‐mediated integrin internalization, where activation by the guanine nucleotide exchange factor BRAG2 couples receptor turnover with cytoskeletal dynamics (see"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": "and."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Moreover, functional studies in viral infection models have underscored the importance of ARF5. In the context of dengue and hepatitis C virus replication, coordinated depletion of ARF4 and ARF5 markedly impairs efficient early secretory pathway functions that are essential for viral particle formation (see"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "and."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": " ARF5’s involvement also extends to the regulation of dense-core vesicle trafficking via its interaction with CAPS1 at the TGN, as well as to maintaining Golgi integrity through its binding to proteins such as GORAB, with mutant forms disrupting this association and altering Golgi localization (see"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": "and."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Finally, ARF5 has been implicated in the regulation of lipid homeostasis; its depletion, along with ARF4, has been shown to affect lipid droplet morphology and cellular triacylglycerol levels, suggesting broader roles in metabolic regulation (see."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": " Collectively, these findings highlight ARF5 as a key regulator in diverse trafficking pathways, whose precise control of vesicular transport integrates signals from various effectors to coordinate secretion, endocytosis, cell division, and metabolic homeostasis.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jan H Reiling, Andrew J Olive, Sumana Sanyal, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb2865"}], "href": "https://doi.org/10.1038/ncb2865"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24185178"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24185178"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jason Lowery, Tomasz Szul, Melanie Styers, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M112.438481"}], "href": "https://doi.org/10.1074/jbc.M112.438481"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23386609"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23386609"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Gilles R X Hickson, Johanne Matheson, Blake Riggs, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Arfophilins are dual Arf/Rab 11 binding proteins that regulate recycling endosome distribution and are related to Drosophila nuclear fallout."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e03-03-0160"}], "href": "https://doi.org/10.1091/mbc.e03-03-0160"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12857874"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12857874"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Tomoo Shiba, Hiroshi Koga, Hye-Won Shin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.0605357103"}], "href": "https://doi.org/10.1073/pnas.0605357103"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17030804"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17030804"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Radim Moravec, Kathryn K Conger, Ryan D'Souza, et al. "}, {"type": "b", "children": [{"type": "t", "text": "BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M112.383117"}], "href": "https://doi.org/10.1074/jbc.M112.383117"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22815487"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22815487"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Lee P Haynes, Mark W Sherwood, Nick J Dolman, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Traffic (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/j.1600-0854.2007.00594.x"}], "href": "https://doi.org/10.1111/j.1600-0854.2007.00594.x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17555535"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17555535"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Mateusz Kudelko, Jean-Baptiste Brault, Kevin Kwok, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Class II ADP-ribosylation factors are required for efficient secretion of dengue viruses."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M111.270579"}], "href": "https://doi.org/10.1074/jbc.M111.270579"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22105072"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22105072"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Rayan Farhat, Karin Séron, Juliette Ferlin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of class II ADP-ribosylation factors as cellular factors required for hepatitis C virus replication."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Microbiol (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/cmi.12572"}], "href": "https://doi.org/10.1111/cmi.12572"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26814617"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26814617"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Tetsushi Sadakata, Yo Shinoda, Yukiko Sekine, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M110.137414"}], "href": "https://doi.org/10.1074/jbc.M110.137414"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20921225"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20921225"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Johannes Egerer, Denise Emmerich, Björn Fischer-Zirnsak, et al. "}, {"type": "b", "children": [{"type": "t", "text": "GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Invest Dermatol (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/jid.2015.192"}], "href": "https://doi.org/10.1038/jid.2015.192"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26000619"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26000619"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Kouhei Takashima, Akina Saitoh, Shohei Hirose, et al. "}, {"type": "b", "children": [{"type": "t", "text": "GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Struct Funct (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1247/csf.11035"}], "href": "https://doi.org/10.1247/csf.11035"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22185782"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22185782"}]}]}]}
Proteins	ARF5_HUMAN
NCBI Gene ID	381
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

ARF5 has 7,272 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 114 datasets.

Click the + buttons to view associations for ARF5 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

ARF5 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by ARF5 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARF5 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARF5 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of ARF5 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of ARF5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of ARF5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARF5 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of ARF5 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ARF5 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ARF5 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of ARF5 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of ARF5 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of ARF5 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with ARF5 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with ARF5 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ARF5 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of ARF5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of ARF5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of ARF5 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing ARF5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing ARF5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with ARF5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with ARF5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of ARF5 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with ARF5 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with ARF5 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by ARF5 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with ARF5 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with ARF5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with ARF5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with ARF5 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at ARF5 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ARF5 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of ARF5 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing ARF5 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of ARF5 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with ARF5 gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing ARF5 from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of ARF5 gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.
	GEO Signatures of Differentially Expressed Genes for Gene Perturbations	gene perturbations changing expression of ARF5 gene from the GEO Signatures of Differentially Expressed Genes for Gene Perturbations dataset.