ARHGAP35 Gene

HGNC Family	Rho GTPase activating proteins (ARHGAP)
Name	Rho GTPase activating protein 35
Description	The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ARHGAP35, which encodes the p190RhoGAP‐A protein, is emerging as a central negative regulator of RhoA activity that integrates diverse mechanical and biochemical cues to control cytoskeletal dynamics, cell adhesion, and migration. Several studies report that ARHGAP35 is recruited to sites of cell–cell and cell–matrix contact where, upon activation by upstream signals such as Rac and kinases (e.g., FAK and Src), it mediates localized inhibition of RhoA. For example, in three‐dimensional fibroblast cultures, caveolin‑1–dependent regulation of p190RhoGAP (ARHGAP35) is critical for force‐dependent matrix remodeling and directional carcinoma cell invasion."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In epithelial cells, p190RhoGAP is recruited to adherens junctions via an interaction with p120‑catenin, a mechanism that facilitates Rac‐dependent antagonism of Rho and thereby promotes junction assembly and cell polarity."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "3", "end_ref": "5"}]}, {"type": "t", "text": " In endothelial cells, FAK-dependent phosphorylation of p190RhoGAP plays a key role in attenuating RhoA activity to restore barrier integrity after permeability challenges."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Moreover, during collective cell migration, Src activation triggered by claudin‑11 signals through p190RhoGAP to suppress RhoA, thereby maintaining stable cell–cell contacts."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": " Finally, integrin clustering induced by cell surface transglutaminase has been shown to diminish Src-mediated p190RhoGAP regulation, leading to enhanced RhoA activity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " Collectively, these findings indicate that ARHGAP35 fulfills an essential role in coordinating the balance between RhoA activation and inhibition in response to a variety of upstream stimuli, thereby orchestrating cell polarity, adhesion dynamics, and migration in both normal physiology and disease.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jacky G Goetz, Susana Minguet, Inmaculada Navarro-Lérida, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2011.05.040"}], "href": "https://doi.org/10.1016/j.cell.2011.05.040"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21729786"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21729786"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Araceli Grande-García, Asier Echarri, Johan de Rooij, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.200701006"}], "href": "https://doi.org/10.1083/jcb.200701006"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17517963"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17517963"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Gregg A Wildenberg, Michael R Dohn, Robert H Carnahan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "p120-catenin and p190RhoGAP regulate cell-cell adhesion by coordinating antagonism between Rac and Rho."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2006.09.046"}], "href": "https://doi.org/10.1016/j.cell.2006.09.046"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17129786"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17129786"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "William D Bradley, Samuel E Hernández, Jeffrey Settleman, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Integrin signaling through Arg activates p190RhoGAP by promoting its binding to p120RasGAP and recruitment to the membrane."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e06-02-0132"}], "href": "https://doi.org/10.1091/mbc.e06-02-0132"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16971514"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16971514"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Alok Tomar, Ssang-Taek Lim, Yangmi Lim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.046870"}], "href": "https://doi.org/10.1242/jcs.046870"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19435801"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19435801"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Michael Holinstat, Nebojsa Knezevic, Michael Broman, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M511248200"}], "href": "https://doi.org/10.1074/jbc.M511248200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16308318"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16308318"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Tadanori Mammoto, Samir M Parikh, Akiko Mammoto, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Angiopoietin-1 requires p190 RhoGAP to protect against vascular leakage in vivo."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M702169200"}], "href": "https://doi.org/10.1074/jbc.M702169200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17562701"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17562701"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Ching-Fei Li, Jia-Yang Chen, Yang-Hui Ho, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Snail-induced claudin-11 prompts collective migration for tumour progression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41556-018-0268-z"}], "href": "https://doi.org/10.1038/s41556-018-0268-z"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30664792"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30664792"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Anna Janiak, Evgeny A Zemskov, Alexey M Belkin "}, {"type": "b", "children": [{"type": "t", "text": "Cell surface transglutaminase promotes RhoA activation via integrin clustering and suppression of the Src-p190RhoGAP signaling pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e05-06-0549"}], "href": "https://doi.org/10.1091/mbc.e05-06-0549"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16452636"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16452636"}]}]}]}
Synonyms	P190A, P190RHOGAP, P190ARHOGAP, GRLF1, GRF-1, P190-A
Proteins	RHG35_HUMAN
NCBI Gene ID	2909
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

ARHGAP35 has 6,920 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 124 datasets.

Click the + buttons to view associations for ARHGAP35 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

ARHGAP35 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by ARHGAP35 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARHGAP35 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARHGAP35 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of ARHGAP35 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of ARHGAP35 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of ARHGAP35 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ARHGAP35 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of ARHGAP35 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ARHGAP35 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ARHGAP35 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of ARHGAP35 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of ARHGAP35 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with ARHGAP35 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with ARHGAP35 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ARHGAP35 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of ARHGAP35 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of ARHGAP35 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of ARHGAP35 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing ARHGAP35 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing ARHGAP35 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing ARHGAP35 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with ARHGAP35 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with ARHGAP35 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	CORUM Protein Complexes	protein complexs containing ARHGAP35 protein from the CORUM Protein Complexes dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of ARHGAP35 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with ARHGAP35 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with ARHGAP35 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by ARHGAP35 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DEPOD Substrates of Phosphatases	phosphatases that dephosphorylate ARHGAP35 protein from the curated DEPOD Substrates of Phosphatases dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with ARHGAP35 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with ARHGAP35 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with ARHGAP35 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with ARHGAP35 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with ARHGAP35 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at ARHGAP35 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ARHGAP35 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of ARHGAP35 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing ARHGAP35 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with ARHGAP35 gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GAD High Level Gene-Disease Associations	diseases associated with ARHGAP35 gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.