{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ARPC5L is an alternatively expressed isoform of the ARPC5 subunit of the seven‐member Arp2/3 complex that plays a pivotal role in regulating branched actin network formation. Arp2/3 complexes harboring ARPC5L exhibit enhanced actin nucleation and generate more stable branched networks compared to complexes containing the canonical ARPC5 isoform; this increased activity is attributed to a partially disordered N‐terminal region of ARPC5L that appears to be an important determinant of complex activity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond its role in dynamic actin assembly, ARPC5L contributes to several cellular processes. In skeletal muscle, an Arp2/3 complex containing ARPC5L, in conjunction with γ‐actin, is required to organize desmin for effective crosslinking of myofibrils during the centripetal movement of nuclei, a process critical for proper myofibre maturation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": " Abnormalities in ARPC5L transcripts have also been implicated in endocrine dysfunction; for example, defective splicing of ARPC5L is associated with isolated growth hormone deficiency, suggesting a role in the restricted function of somatotroph cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n ARPC5L is further embedded within broader cellular networks. It appears as a component of the protein interactome of PKD2, implying a potential role in Golgi-associated transport processes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " In pathological contexts, such as cancer, ARPC5L-containing complexes are found to be upregulated; for instance, increased expression of several Arp2/3 subunits including ARPC5L has been observed in hepatocellular carcinoma, and alterations in ARPC5L phosphorylation correlate with impaired cell migration and adhesion, supporting its involvement in modulating metastatic potential."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Finally, elevated ARPC5L mRNA expression in bovine conceptuses compared to the endometrium suggests that ARPC5L may also participate in mediating cross-talk at the maternal–conceptus interface during the pregnancy recognition period."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": " Collectively, these findings emphasize that ARPC5L is a key modulator of actin cytoskeleton dynamics, influencing cellular motility, structural organization, and various disease-related processes.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jasmine V G Abella, Chiara Galloni, Julien Pernier, et al. 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