{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ASAP1, also known as DDEF1 or AMAP1, is a multidomain Arf GTPase‐activating protein that plays a central role in the regulation of cell adhesion, migration, invasion, and membrane trafficking. Its unique architecture—including BAR, pleckstrin homology (PH), ankyrin repeat, and SH3 domains—enables ASAP1 to integrate signals from tyrosine kinases (such as Src), focal adhesion kinase (FAK) and other adaptor proteins (e.g. CrkL and CIN85) to modulate the dynamic organization of the actin cytoskeleton and focal adhesion assemblies (4, 19, 21). In the context of cancer, elevated ASAP1 expression promotes oncogenic processes by facilitating invadopodia and podosome formation, enhancing cell motility and invasiveness in prostate, breast, and uveal melanomas, among others (1, 6, 10, 12, 15, 22). Furthermore, ASAP1 functions as a scaffold in specialized membrane trafficking events. It regulates integrin recycling to promote cell invasion under EGFR stimulation (11) and plays a critical role in the ciliary targeting of sensory receptors—such as rhodopsin—by coordinating the assembly of Rab11–Rab8 complexes with Arf4 (7, 13, 23). ASAP1 activity is also modulated by post-translational modifications; for instance, Src-mediated phosphorylation can alter its interactions and its GAP activity, while its BAR domain can autoinhibit catalytic activity toward Arf1 (4, 8, 14, 17). In addition, genetic studies indicate that deregulated ASAP1 expression, whether by polymorphisms affecting dendritic cell migration or by loss of miR-203–mediated repression, can contribute to disease progression—not only promoting metastasis in several cancers (1, 9, 12) but also predisposing to conditions such as tuberculosis due to impaired immune cell motility (5). Collectively, these findings highlight ASAP1 as a multifunctional regulator that integrates cytoskeletal remodeling with membrane dynamics to drive invasive behavior and impact cellular differentiation and trafficking.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n "}, {"type": "b", "children": [{"type": "t", "text": "Citations:"}]}, {"type": "t", "text": "<br>\n (1)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ", \n (2)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ", \n (3)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ", \n (4)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ", \n (5)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ", \n (6)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ", \n (7)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": ", \n (8)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": ", \n (9)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": ", \n (10)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": ", \n (11)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": ", \n (12)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "12"}]}, {"type": "t", "text": ", \n (13)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "13"}]}, {"type": "t", "text": ", \n (14)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "14"}]}, {"type": "t", "text": ", \n (15)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "15"}]}, {"type": "t", "text": ", \n (16)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "16"}]}, {"type": "t", "text": ", \n (17)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "17"}]}, {"type": "t", "text": ", \n (18)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "18"}]}, {"type": "t", "text": ", \n (19)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "19"}]}, {"type": "t", "text": ", \n (20)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "20"}]}, {"type": "t", "text": ", \n (21)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "21"}]}, {"type": "t", "text": ", \n (22)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "22"}]}, {"type": "t", "text": ", \n (23)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "23"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Giuditta Viticchiè, Anna Maria Lena, Alessia Latina, et al. 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