{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Aurora B kinase (AURKB) is a central mitotic regulator that ensures accurate chromosome segregation and cytokinesis by modulating multiple substrates and processes. In kinetochores, AURKB phosphorylates key components such as the Ndc80/Hec1 complex to regulate microtubule plus‐end dynamics, thereby promoting error correction and stabilizing proper kinetochore–microtubule attachments under tension."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": " At centromeric regions, AURKB contributes to chromatin reorganization by phosphorylating histone H3 at serine 10, a modification that is part of a “methyl/phospho switch” that displaces chromatin-binding proteins such as HP1, thus facilitating the dynamic structural changes required during mitosis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Moreover, spatial regulation of AURKB activity—exemplified by its formation of a phosphorylation gradient emanating from the spindle midzone during anaphase—provides essential spatial cues that coordinate abscission and prevent premature cytokinesis in the presence of unsegregated chromatin."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " Such a gradient, together with its centromeric enrichment mediated by histone H3 threonine 3 phosphorylation and Survivin binding, ensures correct kinetochore function and maintains spindle checkpoint signaling."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": " Furthermore, formation of the chromosomal passenger complex comprising AURKB, INCENP, and Survivin is vital for its proper localization and activation, which in turn is critical for both chromosome alignment and cytokinesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " Finally, chemical-genetic studies employing selective Aurora kinase inhibitors emphasize that AURKB activity is indispensable for mitotic fidelity and represents an attractive target for anti-proliferative strategies."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": " Collectively, these studies underscore AURKB’s multifaceted role in coupling kinetochore–microtubule dynamics, chromatin modifications, and cytokinetic progression to preserve genomic stability during cell division.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jennifer G DeLuca, Walter E Gall, Claudio Ciferri, et al. 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