{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Recent work has identified BBOF1 as a novel coiled‐coil protein that plays a critical role in the alignment and maintenance of cilia orientation in multiciliated cells (MCCs)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " The expression of BBOF1 is induced early in MCC differentiation under the regulation of the master transcription factor Foxj1, yet its loss does not impede the overall processes of ciliogenesis or cell differentiation. Rather, BBOF1 is essential for ensuring that the numerous motile cilia, organized around basal bodies, maintain a precise planar orientation. In BBOF1‐deficient cells, the normally coordinated ciliary array becomes severely disorganized, compromising the cell's ability to sustain directional fluid flow. Notably, even when exposed to external patterning signals or hydrodynamic forces, the mutant cilia exhibit an inability to preserve their alignment. Conversely, misexpression of BBOF1 can promote cilia alignment even in conditions where cytoskeletal elements such as microtubules and actin filaments are disrupted. Localization studies further indicate that BBOF1 associates with a polar structure adjacent to the basal body, implicating it as a pivotal component in the molecular machinery that governs planar cell polarity in MCCs.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yuan-Hung Chien, Michael E Werner, Jennifer Stubbs, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Bbof1 is required to maintain cilia orientation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Development (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/dev.096727"}], "href": "https://doi.org/10.1242/dev.096727"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23900544"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23900544"}]}]}]}