BUD31 Gene

Name	BUD31 homolog (S. cerevisiae)
Description	Enables nuclear receptor binding activity and nuclear receptor coactivator activity. Involved in mRNA splicing, via spliceosome and positive regulation of androgen receptor activity. Located in chromatin and nucleus. Part of U2-type catalytic step 2 spliceosome. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n BUD31 is emerging as a multifunctional protein with critical roles in both RNA splicing and transcriptional regulation. In several studies, BUD31 has been identified as a core component of the spliceosome that is essential for its proper assembly and catalytic activity, thereby ensuring efficient pre‐mRNA processing. For example, in MYC‐driven cancers the hyperactivation of MYC leads to a surge in pre‐mRNA synthesis that overloads the spliceosome, and cancer cells become highly dependent on spliceosomal factors such as BUD31 for survival and tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In yeast, mutation or deletion of the BUD31 gene causes severe defects in splicing fidelity, abnormal bud formation, and impaired cell cycle transitions, signifying its indispensable role in orchestrating accurate spliceosome dynamics."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "2", "end_ref": "4"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition to its canonical role in pre‐mRNA splicing, BUD31 has been implicated in modulating cancer cell behavior. In aggressive triple‐negative breast cancers, for instance, BUD31 is required to sustain cell migration by contributing to a gene expression program that regulates focal adhesion and extracellular matrix interactions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " Moreover, studies in prostate cancer have demonstrated that BUD31 can function as a co‐regulator of the androgen receptor (AR). By binding via an FxxLF motif, BUD31 suppresses both wild‐type and mutant AR‐mediated transcriptional activity, and its efficacy can be influenced by point mutations in AR that reduce binding affinity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " \n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Overall, BUD31 plays a pivotal role in maintaining RNA processing integrity while also impacting pathways that govern cell migration and transcriptional regulation in cancer. This dual functionality not only underscores the importance of proper spliceosome assembly for cellular homeostasis but also highlights the potential of targeting BUD31‐associated pathways for therapeutic intervention in various cancers.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Tiffany Y-T Hsu, Lukas M Simon, Nicholas J Neill, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The spliceosome is a therapeutic vulnerability in MYC-driven cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nature14985"}], "href": "https://doi.org/10.1038/nature14985"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26331541"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26331541"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Barbara Masciadri, Liliana B Areces, Patrizia Carpinelli, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Characterization of the BUD31 gene of Saccharomyces cerevisiae."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2004.05.228"}], "href": "https://doi.org/10.1016/j.bbrc.2004.05.228"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15303280"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15303280"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Debjani Saha, Shataparna Banerjee, Samirul Bashir, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Context dependent splicing functions of Bud31/Ycr063w define its role in budding and cell cycle progression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2012.06.156"}], "href": "https://doi.org/10.1016/j.bbrc.2012.06.156"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22789856"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22789856"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Debjani Saha, Piyush Khandelia, Raymond T O'Keefe, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Saccharomyces cerevisiae NineTeen complex (NTC)-associated factor Bud31/Ycr063w assembles on precatalytic spliceosomes and improves first and second step pre-mRNA splicing efficiency."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M111.298547"}], "href": "https://doi.org/10.1074/jbc.M111.298547"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22215661"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22215661"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Esmee Koedoot, Michiel Fokkelman, Vasiliki-Maria Rogkoti, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-019-11020-3"}], "href": "https://doi.org/10.1038/s41467-019-11020-3"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31278301"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31278301"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Tianqing Song, Jiazhong Li "}, {"type": "b", "children": [{"type": "t", "text": "New Insights into the Binding Mechanism of Co-regulator BUD31 to AR AF2 Site: Structural Determination and Analysis of the Mutation Effect."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Curr Comput Aided Drug Des (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.2174/1573409915666190502153307"}], "href": "https://doi.org/10.2174/1573409915666190502153307"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31057123"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31057123"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Cheng-Lung Hsu, Jai-Shin Liu, Po-Long Wu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of a new androgen receptor (AR) co-regulator BUD31 and related peptides to suppress wild-type and mutated AR-mediated prostate cancer growth via peptide screening and X-ray structure analysis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Oncol (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molonc.2014.06.009"}], "href": "https://doi.org/10.1016/j.molonc.2014.06.009"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25091737"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25091737"}]}]}]}
Synonyms	YCR063W, G10, CWC14, FSAP17
Proteins	BUD31_HUMAN
NCBI Gene ID	8896
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

BUD31 has 6,711 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 111 datasets.

Click the + buttons to view associations for BUD31 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

BUD31 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of BUD31 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of BUD31 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of BUD31 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of BUD31 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of BUD31 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of BUD31 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of BUD31 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of BUD31 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of BUD31 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of BUD31 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with BUD31 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with BUD31 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of BUD31 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of BUD31 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of BUD31 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of BUD31 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing BUD31 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing BUD31 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing BUD31 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing BUD31 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with BUD31 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with BUD31 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	CORUM Protein Complexes	protein complexs containing BUD31 protein from the CORUM Protein Complexes dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of BUD31 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with BUD31 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with BUD31 gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with BUD31 gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of BUD31 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with BUD31 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with BUD31 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with BUD31 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with BUD31 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with BUD31 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at BUD31 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of BUD31 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of BUD31 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing BUD31 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with BUD31 gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of BUD31 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing BUD31 from the GeneSigDB Published Gene Signatures dataset.