{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n C6orf58—also known as liver‐enriched gene 1 (LEG1)—is a highly conserved, secreted protein that has been identified in a variety of tissues and body fluids across vertebrates. Evolutionary studies reveal that LEG1 homologs exist from bacteria to mammals and, in some species, have diverged into distinct paralogs that appear to be under strong purifying selection, suggesting a conserved role in cellular homeostasis and lipid metabolism."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Proteomic investigations have detected C6orf58/LEG1 in diverse biological contexts. For example, it has been identified in trout ovarian fluid as an uncharacterized protein"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ", and in ram seminal plasma it is one of only a few sperm‐membrane–binding proteins present after exposure to seminal plasma"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ";."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " In murine models, a LEG1 homologue is expressed predominantly in the small intestine rather than in the liver, and studies using a conditional knock‐out support its secretory nature."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " Similarly, LEG1 has been found in the secretome of human adipose‐derived mesenchymal stem cells"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ", and its appearance in the saliva of women during the ovulatory phase"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "reinforces a role in reproductive physiology.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition to its putative involvement in lipid homeostasis and intercellular signaling, differential abundance of C6orf58 has been observed in clinical settings. Elevated or altered levels of this protein have been reported in the sputum of patients with early-stage chronic obstructive pulmonary disease"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": "and in renal cell carcinoma tissue, where it emerged as a highly differential marker."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " These findings collectively suggest that C6orf58/LEG1 may contribute to processes such as cellular differentiation, metabolism, and reproduction while also holding promise as a diagnostic biomarker.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Although its precise molecular functions remain to be fully elucidated, current evidence indicates that C6orf58/LEG1 participates in diverse biological processes—including lipid metabolism, reproductive function, and possibly host responses in disease—warranting further investigation.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yanna Dang, Jin-Yang Wang, Chen Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Evolutionary and Molecular Characterization of liver-enriched gene 1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-020-61208-7"}], "href": "https://doi.org/10.1038/s41598-020-61208-7"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32144352"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32144352"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Joanna Nynca, Georg J Arnold, Thomas Fröhlich, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Shotgun proteomics of rainbow trout ovarian fluid."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Reprod Fertil Dev (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1071/RD13224"}], "href": "https://doi.org/10.1071/RD13224"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25482144"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25482144"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "C Soleilhavoup, G Tsikis, V Labas, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Proteomics (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jprot.2014.07.007"}], "href": "https://doi.org/10.1016/j.jprot.2014.07.007"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25053255"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25053255"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Taylor Pini, Tamara Leahy, Clement Soleilhavoup, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Proteome Res (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1021/acs.jproteome.6b00530"}], "href": "https://doi.org/10.1021/acs.jproteome.6b00530"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27636150"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27636150"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Zhi-Hui Zhu, Min-Jie Hu, Chang-Qing Chang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "[Analysis of expression pattern of zebrafish leg1 homologus gene mu-leg1 in mouse]."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Yi Chuan (2012)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23017459"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23017459"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of the Proteins Secreted by Human Adipose-Derived Mesenchymal Stem Cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Transplant (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1177/0963689718795096"}], "href": "https://doi.org/10.1177/0963689718795096"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30226075"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30226075"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Ganesan Saibaba, Durairaj Rajesh, Subramanian Muthukumar, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Salivary Proteome Profile of Women during Fertile Phase of Menstrual Cycle as Characterized by Mass Spectrometry."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gynecol Minim Invasive Ther (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4103/GMIT.GMIT_78_20"}], "href": "https://doi.org/10.4103/GMIT.GMIT_78_20"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34909380"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34909380"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Bjoern Titz, Alain Sewer, Thomas Schneider, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Alterations in the sputum proteome and transcriptome in smokers and early-stage COPD subjects."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Proteomics (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jprot.2015.08.009"}], "href": "https://doi.org/10.1016/j.jprot.2015.08.009"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26306861"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26306861"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Francesca Raimondo, Claudia Salemi, Clizia Chinello, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biosyst (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1039/c2mb05390j"}], "href": "https://doi.org/10.1039/c2mb05390j"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22315040"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22315040"}]}]}]}