CDC25C Gene

HGNC Family	Protein phosphatase 1 regulatory subunits (PPP1R), Phosphatases
Name	cell division cycle 25C
Description	This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n CDC25C is a dual‐specificity phosphatase that plays a pivotal role in triggering mitotic entry by dephosphorylating and thereby activating cyclin‐dependent kinases. Its activity is tightly regulated to ensure proper cell cycle progression and a robust DNA damage response. In cells exposed to genotoxic stress, Chk1 phosphorylates CDC25C at serine-216, which creates a binding site for 14-3-3 proteins. This interaction sequesters CDC25C in the cytoplasm and prevents premature activation of the Cdc2–cyclin B complex, thereby enforcing the G2/M checkpoint."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": " Furthermore, CDC25C is also subject to regulatory phosphorylation by Plk1; phosphorylation at serine-198 promotes its rapid nuclear translocation during prophase, which is required for proper mitotic progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " In addition to these phosphorylation‐based mechanisms, CDC25C levels and function are modulated by other post-translational modifications. For instance, aberrant O-GlcNAcylation can lead to transcriptional and protein-level downregulation of CDC25C, resulting in G2/M arrest in cancer cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " Likewise, inhibition of deubiquitylating enzymes (DUBs) has been associated with decreased CDC25C protein levels in multiple myeloma models, further linking its regulation to cell cycle arrest and apoptotic induction."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Finally, the specificity in CDC25C regulation is underscored by its selective association with certain 14-3-3 isoforms, such as 14-3-3σ, which determines distinct ligand-binding properties and thereby fine-tunes checkpoint control."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": " Collectively, these studies highlight how CDC25C integrates multiple signaling inputs—ranging from kinase-mediated phosphorylation to modifications that affect protein stability—to control the critical G2/M transition, with deregulation of these pathways contributing to tumorigenesis and offering potential avenues for therapeutic intervention."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}, {"type": "fg_f", "ref": "8"}, {"type": "fg_fs", "start_ref": "4", "end_ref": "7"}, {"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Y Sanchez, C Wong, R S Thoma, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (1997)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.277.5331.1497"}], "href": "https://doi.org/10.1126/science.277.5331.1497"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "9278511"}], "href": "https://pubmed.ncbi.nlm.nih.gov/9278511"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Pavel A Savitsky, Toren Finkel "}, {"type": "b", "children": [{"type": "t", "text": "Redox regulation of Cdc25C."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M201589200"}], "href": "https://doi.org/10.1074/jbc.M201589200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11925443"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11925443"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "R P Sahu, S Batra, S K Srivastava "}, {"type": "b", "children": [{"type": "t", "text": "Activation of ATM/Chk1 by curcumin causes cell cycle arrest and apoptosis in human pancreatic cancer cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Br J Cancer (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/sj.bjc.6605039"}], "href": "https://doi.org/10.1038/sj.bjc.6605039"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19401701"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19401701"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Fumiko Toyoshima-Morimoto, Eri Taniguchi, Eisuke Nishida "}, {"type": "b", "children": [{"type": "t", "text": "Plk1 promotes nuclear translocation of human Cdc25C during prophase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO Rep (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/embo-reports/kvf069"}], "href": "https://doi.org/10.1093/embo-reports/kvf069"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11897663"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11897663"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Zihao Wang, Namrata D Udeshi, Chad Slawson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Signal (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/scisignal.2000526"}], "href": "https://doi.org/10.1126/scisignal.2000526"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20068230"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20068230"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Ze Tian, Padraig D'Arcy, Xin Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A novel small molecule inhibitor of deubiquitylating enzyme USP14 and UCHL5 induces apoptosis in multiple myeloma and overcomes bortezomib resistance."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2013-05-500033"}], "href": "https://doi.org/10.1182/blood-2013-05-500033"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24319254"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24319254"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Erik W Wilker, Robert A Grant, Stephen C Artim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A structural basis for 14-3-3sigma functional specificity."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M500982200"}], "href": "https://doi.org/10.1074/jbc.M500982200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15731107"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15731107"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Ronit I Yarden, Sherly Pardo-Reoyo, Magda Sgagias, et al. "}, {"type": "b", "children": [{"type": "t", "text": "BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Genet (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ng837"}], "href": "https://doi.org/10.1038/ng837"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11836499"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11836499"}]}]}]}
Synonyms	CDC25, PPP1R60
Proteins	MPIP3_HUMAN
NCBI Gene ID	995
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

CDC25C has 10,256 functional associations with biological entities spanning 9 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 131 datasets.

Click the + buttons to view associations for CDC25C from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

CDC25C Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of CDC25C gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of CDC25C gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of CDC25C gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of CDC25C gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CDC25C gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	Biocarta Pathways	pathways involving CDC25C protein from the Biocarta Pathways dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of CDC25C gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CDC25C gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CDC25C gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of CDC25C gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of CDC25C gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of CDC25C gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Gene Mutation Profiles	cell lines with CDC25C gene mutations from the CCLE Cell Line Gene Mutation Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with CDC25C protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with CDC25C gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CDC25C gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of CDC25C gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of CDC25C gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of CDC25C gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of CDC25C gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing CDC25C protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing CDC25C protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing CDC25C protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with CDC25C protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with CDC25C protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with CDC25C gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with CDC25C gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with CDC25C gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of CDC25C protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by CDC25C gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DEPOD Substrates of Phosphatases	phosphatases that dephosphorylate CDC25C protein from the curated DEPOD Substrates of Phosphatases dataset.
	DGIdb Drug Targets 2026	interacting drugs for CDC25C protein from the DGIdb Drug Targets 2026 dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with CDC25C gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with CDC25C gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with CDC25C gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with CDC25C gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with CDC25C gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at CDC25C gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CDC25C gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of CDC25C gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.