{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n CEP295 is emerging as a conserved, multifunctional protein essential for the maturation and conversion of centrioles into fully competent centrosomes. Several independent studies have shown that CEP295 is recruited early during procentriole assembly at the proximal centriole wall, where it plays a pivotal role in driving “centriole‐to‐centrosome conversion” by acting as a scaffold that links inner centriolar components (such as Cep135) with outer elements (like Cep192 and Asterless/Cep152) that are required for pericentriolar material (PCM) recruitment and microtubule‐organizing activity ."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition, CEP295 is critical for centriole elongation and stability. It is necessary for building the distal half of centrioles by recruiting downstream proteins such as POC5 and POC1B and promoting post‐translational modifications of centriolar microtubules, such as acetylation and glutamylation, which in turn contribute to centriole integrity and proper length control ."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n CEP295’s function is further integrated within a wider network of centriolar components: upstream regulators including proteins such as RTTN and PPP1R35 help establish the correct localization and loading of CEP295, ensuring that the nascent centriole can mature into a functional mother centriole that is competent for PCM recruitment and centrosomal activity ."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Moreover, studies in Drosophila, where the CEP295 homolog Ana1 operates, indicate that CEP295/Ana1 is also involved in recruiting Polo-like kinase to mother centrioles, thereby contributing specifically to mitotic PCM assembly and centriole elongation during G2 ."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, these findings define CEP295 as a central mediator of centriole maturation, coordinating the assembly of distal centriolar structures and organizing essential interactions for centrosome formation and function. The protein’s activity not only ensures the stability and elongation of centrioles but also underpins the conversion of daughter centrioles into fully functional centrosomes required for accurate spindle formation and cell division.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Denisse Izquierdo, Won-Jing Wang, Kunihiro Uryu, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncomms12567"}], "href": "https://doi.org/10.1038/ncomms12567"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27562453"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27562453"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Ching-Wen Chang, Wen-Bin Hsu, Jhih-Jie Tsai, et al. 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