{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n CEP55 is a multifunctional centrosomal and midbody‐associated protein that plays a central role in the final steps of cell division. It functions as a molecular scaffold to recruit components of the endosomal sorting complexes required for transport (ESCRT), including the ESCRT‐I subunit Tsg101 and the ESCRT‐associated protein ALIX, to the midbody. Such recruitment is essential for mediating the membrane fission (abscission) event that physically separates daughter cells—a process that shares key mechanistic parallels with viral budding (for example, HIV‐1 release)."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n During mitotic progression, CEP55 is first localized to the centrosome and is then released and phosphorylated—events mediated by kinases such as Plk1 and Cdk1—that promote its accumulation at the midbody. Here, it interacts with centralspindlin and bundles microtubules to maintain midbody integrity, thereby ensuring proper abscission."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "4", "end_ref": "6"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n CEP55’s binding interfaces are finely regulated, as evidenced by its competitive interactions with ALIX and Tsg101, and aberrations in these interactions—for instance, when disrupted by mutant BRCA2 or by germ cell–specific proteins like TEX14—can compromise midbody architecture or prevent abscission altogether. In male germ cells, binding of TEX14 to CEP55 blocks the recruitment of ALIX and Tsg101, thereby converting CEP55 from an abscission factor into a stabilizer of intercellular bridges."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Furthermore, deregulated CEP55 expression is linked to genomic instability and cancer progression. Overexpression of CEP55 appears to protect aneuploid cells from the lethal consequences of aberrant mitosis and has been correlated with aggressive tumor phenotypes, thereby presenting a promising prognostic marker as well as a potential therapeutic target in anti‐mitotic strategies."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, these studies establish CEP55 as a key mediator of cytokinesis through orchestrating ESCRT recruitment and midbody stability, which is critically important not only for faithful cell division but also for maintaining genomic integrity in normal and cancer cells.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jez G Carlton, Juan Martin-Serrano "}, {"type": "b", "children": [{"type": "t", "text": "Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1143422"}], "href": "https://doi.org/10.1126/science.1143422"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17556548"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17556548"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Eiji Morita, Virginie Sandrin, Hyo-Young Chung, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Dev Cell (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.devcel.2005.09.003"}], "href": "https://doi.org/10.1016/j.devcel.2005.09.003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16198290"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16198290"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Wei-meng Zhao, Akiko Seki, Guowei Fang "}, {"type": "b", "children": [{"type": "t", "text": "Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e06-01-0015"}], "href": "https://doi.org/10.1091/mbc.e06-01-0015"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16790497"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16790497"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Isabel Martinez-Garay, Amin Rustom, Hans-Hermann Gerdes, et al. 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