{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n CFAP161 is an evolutionarily conserved protein whose expression is tightly linked to the presence of motile cilia. In studies using both Xenopus and mouse models, CFAP161 was identified as a target of the transcription factor FOXJ1, and its expression correlates with motile cilia formation. In Xenopus, tagged CFAP161 localizes to basal bodies, whereas in mice it is found within the axoneme, suggesting that it is an integral component of ciliary structure and may be involved in the assembly or stability of the ciliary apparatus."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Interestingly, genetic disruption of CFAP161 did not produce overt motile cilia dysfunction, implying that compensatory mechanisms—possibly involving the dysregulation of genes related to microtubules, motor activity, and inner dynein components—may mitigate the loss of CFAP161 function.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Additional evidence for the role of CFAP161 in cilia and flagella function comes from sperm proteome analyses. Here, CFAP161 was identified among a group of proteins that undergo differential carbonylation following cryopreservation. This oxidative modification, which affects proteins involved in cytoskeletal and flagella organization as well as in capacitation processes, underscores the potential impact of environmental stress on CFAP161 function in the context of sperm motility and fertilization."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Together, these findings suggest that CFAP161 is a cilia‐ and flagella‐associated protein that may contribute to the structural or regulatory integrity of motile cilia and flagella. However, the absence of overt phenotypes upon gene disruption suggests that its role may be partially redundant or compensated by parallel pathways.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Anja Beckers, Franziska Fuhl, Tim Ott, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The highly conserved FOXJ1 target CFAP161 is dispensable for motile ciliary function in mouse and Xenopus."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-021-92495-3"}], "href": "https://doi.org/10.1038/s41598-021-92495-3"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34172766"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34172766"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Agnieszka Mostek, Mariola Aleksandra Dietrich, Mariola Słowińska, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Cryopreservation of bull semen is associated with carbonylation of sperm proteins."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Theriogenology (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.theriogenology.2017.01.011"}], "href": "https://doi.org/10.1016/j.theriogenology.2017.01.011"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28237350"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28237350"}]}]}]}