CLSPN Gene

Name	claspin
Description	The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Claspin is an essential adaptor protein that mediates the activation of the checkpoint kinase Chk1 by ATR in response to replication stress and DNA damage. By acting as a molecular scaffold at stalled replication forks, Claspin facilitates the assembly of key DNA damage‐response complexes—including ATR, BRCA1, Tipin, and Timeless—to promote efficient Chk1 phosphorylation and ensure proper cell cycle arrest and repair processes."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "4"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n The function of Claspin is tightly regulated by phosphorylation and ubiquitin‐mediated turnover. Its phosphorylation by ATR and additional kinases is necessary for Chk1 activation, whereas timely degradation—mediated by E3 ubiquitin ligases such as SCF<sup>βTrCP</sup> in concert with Plk1—is required for proper checkpoint termination and cell cycle re‐entry. Notably, deubiquitylating enzymes like USP7 counteract this degradation to sustain Claspin’s activity under genotoxic stress."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "5", "end_ref": "8"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition to its role in checkpoint signal transduction, Claspin contributes to maintaining high rates of replication fork progression during normal S phase and enhances cellular tolerance to oncogene‐induced replication stress, thereby safeguarding genome integrity. Such multifaceted regulatory roles underscore the central importance of Claspin in the DNA damage response and highlight its potential as a target for therapeutic intervention in cancer."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Claudia Christiano Silva Chini, Junjie Chen "}, {"type": "b", "children": [{"type": "t", "text": "Human claspin is required for replication checkpoint control."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M301136200"}], "href": "https://doi.org/10.1074/jbc.M301136200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12766152"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12766152"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Angelo Peschiaroli, N Valerio Dorrello, Daniele Guardavaccaro, et al. "}, {"type": "b", "children": [{"type": "t", "text": "SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molcel.2006.06.013"}], "href": "https://doi.org/10.1016/j.molcel.2006.06.013"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16885022"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16885022"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Shiaw-Yih Lin, Kaiyi Li, Grant S Stewart, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.0401847101"}], "href": "https://doi.org/10.1073/pnas.0401847101"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15096610"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15096610"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Eva Petermann, Thomas Helleday, Keith W Caldecott "}, {"type": "b", "children": [{"type": "t", "text": "Claspin promotes normal replication fork rates in human cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e07-10-1035"}], "href": "https://doi.org/10.1091/mbc.e07-10-1035"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18353973"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18353973"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Niels Mailand, Simon Bekker-Jensen, Jiri Bartek, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molcel.2006.06.016"}], "href": "https://doi.org/10.1016/j.molcel.2006.06.016"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16885021"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16885021"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Ivan Mamely, Marcel Atm van Vugt, Veronique A J Smits, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Curr Biol (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cub.2006.08.026"}], "href": "https://doi.org/10.1016/j.cub.2006.08.026"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16934469"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16934469"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Florian Bassermann, David Frescas, Daniele Guardavaccaro, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2008.05.043"}], "href": "https://doi.org/10.1016/j.cell.2008.05.043"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18662541"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18662541"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Helene Faustrup, Simon Bekker-Jensen, Jiri Bartek, et al. "}, {"type": "b", "children": [{"type": "t", "text": "USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.200807137"}], "href": "https://doi.org/10.1083/jcb.200807137"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19124652"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19124652"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Julien N Bianco, Valérie Bergoglio, Yea-Lih Lin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-019-08886-8"}], "href": "https://doi.org/10.1038/s41467-019-08886-8"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30796221"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30796221"}]}]}]}
Proteins	CLSPN_HUMAN
NCBI Gene ID	63967
API
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

CLSPN has 8,514 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 128 datasets.

Click the + buttons to view associations for CLSPN from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

CLSPN Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CLSPN gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of CLSPN gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of CLSPN gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of CLSPN gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of CLSPN gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CLSPN gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of CLSPN gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CLSPN gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CLSPN gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of CLSPN gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of CLSPN gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with CLSPN protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with CLSPN gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CLSPN gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of CLSPN gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of CLSPN gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of CLSPN gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing CLSPN protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of CLSPN gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing CLSPN protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing CLSPN protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with CLSPN protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with CLSPN protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of CLSPN gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with CLSPN gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with CLSPN gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with CLSPN gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with CLSPN gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of CLSPN protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with CLSPN gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with CLSPN gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with CLSPN gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with CLSPN gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at CLSPN gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CLSPN gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of CLSPN gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing CLSPN from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with CLSPN gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GAD High Level Gene-Disease Associations	diseases associated with CLSPN gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of CLSPN gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.