CTIF Gene

Name	CBP80/20-dependent translation initiation factor
Description	CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs, the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins, including EIF4G (MIM 600495), and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al., 2009 [PubMed 19648179]).[supplied by OMIM, Dec 2009]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n CTIF (CBP80/20‐dependent translation initiation factor) is a pivotal regulator of a specialized, cap‐dependent translation pathway that operates on newly exported mRNAs. In this mode of translation, CTIF directly interacts with the nuclear cap‐binding protein CBP80 and the ribosome‐recruiting factor eIF3g to bridge the cap‐binding complex with the 40S ribosomal subunit, thereby ensuring efficient translation initiation that is tightly coupled to mRNA quality control, including nonsense‐mediated decay (NMD)."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n CTIF’s activity is spatially regulated: it is sequestered in the perinuclear region via interaction with the helicase DDX19B until it is transferred to CBP80 on freshly exported mRNAs, a process that confines translation initiation to a defined subcellular locale. In addition to its canonical role in translation, CTIF participates in specialized pathways. For instance, it interfaces with the stem‐loop binding protein (SLBP) during histone mRNA translation"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ", and it also interacts with viral regulatory proteins—such as HIV Rev—to negatively modulate Gag synthesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond translation initiation, CTIF has been identified as a novel target of poly(ADP‐ribose)ylation by tankyrase enzymes, linking it to the regulation of centrosomal satellite distribution."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Moreover, under proteotoxic stress conditions, CTIF becomes part of a protein quality control complex with eEF1A1 and DCTN1 that facilitates the targeting of misfolded polypeptides to the aggresome, thereby connecting translational control with degradation pathways."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In summary, CTIF functions as a multifunctional adaptor that not only drives a distinct mode of cap‐dependent translation initiation on newly exported mRNAs but also integrates mRNA surveillance and quality control processes. Its roles in histone mRNA translation, antiviral responses, centrosome dynamics, and aggresome formation underscore its importance in regulating gene expression and maintaining cellular homeostasis.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Kyoung Mi Kim, Hana Cho, Kobong Choi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genes Dev (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1101/gad.1823409"}], "href": "https://doi.org/10.1101/gad.1823409"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19648179"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19648179"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Junho Choe, Nara Oh, Sungjin Park, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M111.327528"}], "href": "https://doi.org/10.1074/jbc.M111.327528"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22493286"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22493286"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Yeonkyoung Park, Joori Park, Hyun Jung Hwang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF-DDX19B interaction."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nucleic Acids Res (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/nar/gkab579"}], "href": "https://doi.org/10.1093/nar/gkab579"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34232997"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34232997"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Junho Choe, Kyoung Mi Kim, Sungjin Park, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Rapid degradation of replication-dependent histone mRNAs largely occurs on mRNAs bound by nuclear cap-binding proteins 80 and 20."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nucleic Acids Res (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/nar/gks1196"}], "href": "https://doi.org/10.1093/nar/gks1196"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23234701"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23234701"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Francisco García-de-Gracia, Aracelly Gaete-Argel, Sebastián Riquelme-Barrios, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CBP80/20-dependent translation initiation factor (CTIF) inhibits HIV-1 Gag synthesis by targeting the function of the viral protein Rev."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "RNA Biol (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/15476286.2020.1832375"}], "href": "https://doi.org/10.1080/15476286.2020.1832375"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33103564"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33103564"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Dragomir B Krastev, Stephen J Pettitt, James Campbell, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-018-04466-4"}], "href": "https://doi.org/10.1038/s41467-018-04466-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29789535"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29789535"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Yeonkyoung Park, Joori Park, Yoon Ki Kim "}, {"type": "b", "children": [{"type": "t", "text": "Crosstalk between translation and the aggresome-autophagy pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Autophagy (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/15548627.2017.1358849"}], "href": "https://doi.org/10.1080/15548627.2017.1358849"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28837386"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28837386"}]}]}]}
Synonyms	GM672, KIAA0427
Proteins	CTIF_HUMAN
NCBI Gene ID	9811
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

CTIF has 6,187 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 114 datasets.

Click the + buttons to view associations for CTIF from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

CTIF Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CTIF gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of CTIF gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of CTIF gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of CTIF gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of CTIF gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CTIF gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of CTIF gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CTIF gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CTIF gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of CTIF gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of CTIF gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of CTIF gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with CTIF protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with CTIF gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CTIF gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of CTIF gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of CTIF gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of CTIF gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing CTIF protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of CTIF gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing CTIF protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing CTIF protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing CTIF protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with CTIF protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with CTIF protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of CTIF gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with CTIF gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with CTIF gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with CTIF gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of CTIF protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by CTIF gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores	diseases associated with CTIF gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with CTIF gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with CTIF gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with CTIF gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with CTIF gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with CTIF gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at CTIF gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CTIF gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of CTIF gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.