EP300 Gene

HGNC Family	Chromatin-modifying enzymes
Name	E1A binding protein p300
Description	This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n EP300 (also known as p300) is a versatile transcriptional coactivator with intrinsic histone acetyltransferase activity that plays a central role in regulating gene expression through chromatin remodeling and direct acetylation of transcription factors. Its activity promotes the formation of transcriptionally competent chromatin by acetylating histones and non‐histone substrates, thereby facilitating the recruitment and stabilization of transcriptional complexes. For example, in B‐cell lymphomas, inactivating mutations in EP300 (and its close homologue CREBBP) disrupt the acetylation of key oncoproteins and tumor suppressors, contributing to aberrant gene expression that drives lymphomagenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Under hypoxic conditions, EP300 is recruited to complexes containing hypoxia‐inducible factor (HIF)–1α, where its acetyltransferase activity is critical for full activation of hypoxia-responsive genes."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "2", "end_ref": "4"}]}, {"type": "t", "text": " In the immune system, EP300 is similarly essential: during T helper 17 differentiation, it is recruited—via complexes that include HIF–1α and the transcription factor RORγt—to the IL-17 promoter, thereby enhancing the transcription of signature inflammatory genes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " Moreover, EP300-mediated acetylation of STAT3 at lysine 685 promotes its dimerization and transcriptional activity, which is vital for driving cell growth and progression in response to cytokine signals."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond these roles, EP300 (in concert with the related coactivator CBP) is responsible for establishing specific histone acetylation marks—such as H3K18 and H3K27 acetylation—that are indispensable for the activation of nuclear receptor-dependent transcriptional programs."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": " In vascular endothelial cells, the recruitment of CBP/EP300 by transcription factors like KLF2 is a key step in inducing the expression of vasoprotective genes such as endothelial nitric oxide synthase."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": " Similarly, in the context of inflammatory responses, EP300 acetylates the NF-κB p65 subunit, an event that modulates its DNA binding and facilitates proper temporal control of NF-κB-dependent gene expression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " EP300 also contributes to the stabilization and activation of the tumor suppressor p53 by acetylating it, thereby enhancing p53’s ability to regulate its target genes involved in cell cycle arrest and apoptosis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": " Finally, interplay between EP300 and other chromatin regulators is highlighted by evidence that cross-talk between histone methylation and acetylation events—mediated in part through EP300 activity—is essential for fine-tuning transcriptional outcomes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n In summary, EP300 integrates diverse environmental and metabolic signals by acetylating both histone and non-histone proteins. This activity is critical for the coordination of hypoxic, inflammatory, metabolic, and proliferative responses, underscoring its central role in normal physiology as well as in disease pathogenesis.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ryan D Morin, Maria Mendez-Lago, Andrew J Mungall, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Frequent mutation of histone-modifying genes in non-Hodgkin lymphoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nature10351"}], "href": "https://doi.org/10.1038/nature10351"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21796119"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21796119"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "David Lando, Daniel J Peet, Dean A Whelan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1068592"}], "href": "https://doi.org/10.1126/science.1068592"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11823643"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11823643"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Weibo Luo, Hongxia Hu, Ryan Chang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2011.03.054"}], "href": "https://doi.org/10.1016/j.cell.2011.03.054"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21620138"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21620138"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Aifu Lin, Chunlai Li, Zhen Xing, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb3295"}], "href": "https://doi.org/10.1038/ncb3295"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26751287"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26751287"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Eric V Dang, Joseph Barbi, Huang-Yu Yang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Control of T(H)17/T(reg) balance by hypoxia-inducible factor 1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2011.07.033"}], "href": "https://doi.org/10.1016/j.cell.2011.07.033"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21871655"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21871655"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Zheng-Long Yuan, Ying-Jie Guan, Devasis Chatterjee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Stat3 dimerization regulated by reversible acetylation of a single lysine residue."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1105166"}], "href": "https://doi.org/10.1126/science.1105166"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15653507"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15653507"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Qihuang Jin, Li-Rong Yu, Lifeng Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO J (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/emboj.2010.318"}], "href": "https://doi.org/10.1038/emboj.2010.318"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21131905"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21131905"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Sucharita SenBanerjee, Zhiyong Lin, G Brandon Atkins, et al. "}, {"type": "b", "children": [{"type": "t", "text": "KLF2 Is a novel transcriptional regulator of endothelial proinflammatory activation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Exp Med (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1084/jem.20031132"}], "href": "https://doi.org/10.1084/jem.20031132"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15136591"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15136591"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Rosemary Kiernan, Vanessa Brès, Raymond W M Ng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Post-activation turn-off of NF-kappa B-dependent transcription is regulated by acetylation of p65."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M209572200"}], "href": "https://doi.org/10.1074/jbc.M209572200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12419806"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12419806"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Chao Dai, Wei Gu "}, {"type": "b", "children": [{"type": "t", "text": "p53 post-translational modification: deregulated in tumorigenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Trends Mol Med (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molmed.2010.09.002"}], "href": "https://doi.org/10.1016/j.molmed.2010.09.002"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20932800"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20932800"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "H Wang, R Cao, L Xia, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2001)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/s1097-2765(01)00405-1"}], "href": "https://doi.org/10.1016/s1097-2765(01"}, {"type": "t", "text": "00405-1) PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11779497"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11779497"}]}]}]}
Synonyms	RSTS2, P300, KAT3B
Proteins	EP300_HUMAN
NCBI Gene ID	2033
API
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

EP300 has 14,380 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 144 datasets.

Click the + buttons to view associations for EP300 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

EP300 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by EP300 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of EP300 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of EP300 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of EP300 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of EP300 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of EP300 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of EP300 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	Biocarta Pathways	pathways involving EP300 protein from the Biocarta Pathways dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of EP300 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of EP300 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of EP300 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of EP300 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of EP300 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of EP300 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Gene Mutation Profiles	cell lines with EP300 gene mutations from the CCLE Cell Line Gene Mutation Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with EP300 protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of EP300 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of EP300 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of EP300 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	ClinVar Gene-Phenotype Associations	phenotypes associated with EP300 gene from the curated ClinVar Gene-Phenotype Associations dataset.
	ClinVar Gene-Phenotype Associations 2025	phenotypes associated with EP300 gene from the curated ClinVar Gene-Phenotype Associations 2025 dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing EP300 protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of EP300 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing EP300 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing EP300 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing EP300 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing EP300 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with EP300 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with EP300 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	CORUM Protein Complexes	protein complexs containing EP300 protein from the CORUM Protein Complexes dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of EP300 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with EP300 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with EP300 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with EP300 gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with EP300 gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DISEASES Curated Gene-Disease Association Evidence Scores	diseases involving EP300 gene from the DISEASES Curated Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Curated Gene-Disease Association Evidence Scores 2025	diseases involving EP300 gene from the DISEASES Curated Gene-Disease Association Evidence Scores 2025 dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores	diseases associated with EP300 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with EP300 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with EP300 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.