{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n GAGE12B belongs to the GAGE family, a group of cancer/testis antigens normally restricted in expression to the testis but aberrantly activated in various tumors. Such genes are challenging to quantify by RNA‐Seq due to their membership in larger, highly homologous gene families that produce ambiguously mapped reads, which can lead to an underestimation of their expression levels in disease studies."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " \n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In the cancer context, members of the GAGE family – including GAGE12B – have attracted attention because they are preferentially expressed in cancer stem‐like cells (CSCs) or cancer‐initiating cells (CICs), which are known for their roles in tumor initiation, progression, and therapy resistance."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " This expression pattern renders them promising candidates for targeted immunotherapy, where their limited expression in normal tissues (apart from immune‐privileged sites such as the testis) and their immunogenic properties could be exploited to elicit cytotoxic T‐cell responses against tumors."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Overall, GAGE12B is emerging as a molecule of interest not only because its expression may be underestimated by conventional transcriptomic approaches, but also because it represents a potential CSC antigen that could be harnessed for cancer immunotherapy. Understanding its true expression level and function in tumor biology will be key to developing strategies aimed at targeting the resilient subpopulation of cancer cells that drive disease relapse and progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Christelle Robert, Mick Watson "}, {"type": "b", "children": [{"type": "t", "text": "Errors in RNA-Seq quantification affect genes of relevance to human disease."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genome Biol (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s13059-015-0734-x"}], "href": "https://doi.org/10.1186/s13059-015-0734-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26335491"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26335491"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "R Yamada, A Takahashi, T Torigoe, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Preferential expression of cancer/testis genes in cancer stem-like cells: proposal of a novel sub-category, cancer/testis/stem gene."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Tissue Antigens (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/tan.12113"}], "href": "https://doi.org/10.1111/tan.12113"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23574628"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23574628"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Yoshihiko Hirohashi, Toshihiko Torigoe, Tomohide Tsukahara, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Immune responses to human cancer stem-like cells/cancer-initiating cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Sci (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/cas.12830"}], "href": "https://doi.org/10.1111/cas.12830"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26440127"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26440127"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Shilpa Ravindran, Saad Rasool, Cristina Maccalli "}, {"type": "b", "children": [{"type": "t", "text": "The Cross Talk between Cancer Stem Cells/Cancer Initiating Cells and Tumor Microenvironment: The Missing Piece of the Puzzle for the Efficient Targeting of these Cells with Immunotherapy."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Microenviron (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s12307-019-00233-1"}], "href": "https://doi.org/10.1007/s12307-019-00233-1"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31758404"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31758404"}]}]}]}