{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nNCBP3 is a novel cap‐binding protein that functions as part of an alternative nuclear cap‐binding complex (CBC) with NCBP1. This alternative CBC is capable of binding the 5′‐cap of RNA polymerase II transcripts and aids in mRNA biogenesis by promoting polyadenylated RNA export under normal conditions; however, its role becomes particularly crucial during cellular stress such as viral infection, when the canonical NCBP2-dependent complex is insufficient."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "NCBP3 also exhibits a distinct interaction profile compared to its canonical partners by preferentially associating with components of the Exon Junction Complex (EJC) and the TREX complex. Through these interactions, NCBP3 stabilizes multi-exonic transcripts by competing with RNA degradation factors and thereby facilitates proper mRNA processing and nuclear export."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "Further quantitative interactome screenings have refined our understanding of NCBP3’s associations within ribonucleoprotein assemblies, underscoring its role in mRNA fate decisions while interacting selectively with factors influencing RNA maturation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "In addition to its general role in RNA processing, NCBP3 has been implicated in glioma progression. It is upregulated in glioma tissues and cells, where it binds and stabilizes the small nucleolar RNA host gene 6 (SNHG6). This interaction promotes the repression of the transcription factor GBX2 via polycomb repressive complex 2-mediated H3K27me3 modification, ultimately leading to enhanced expression of oncogenic targets such as FLOT1 and facilitating malignant progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Anna Gebhardt, Matthias Habjan, Christian Benda, et al. "}, {"type": "b", "children": [{"type": "t", "text": "mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncomms9192"}], "href": "https://doi.org/10.1038/ncomms9192"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26382858"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26382858"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Yuhui Dou, Isabelle Barbosa, Hua Jiang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NCBP3 positively impacts mRNA biogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nucleic Acids Res (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/nar/gkaa744"}], "href": "https://doi.org/10.1093/nar/gkaa744"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32960271"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32960271"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Yuhui Dou, Svetlana Kalmykova, Maria Pashkova, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Affinity proteomic dissection of the human nuclear cap-binding complex interactome."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nucleic Acids Res (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/nar/gkaa743"}], "href": "https://doi.org/10.1093/nar/gkaa743"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32960270"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32960270"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Xiwen Li, Fangfang Zhang, Jun Ma, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "RNA Biol (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/15476286.2020.1790140"}], "href": "https://doi.org/10.1080/15476286.2020.1790140"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32618493"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32618493"}]}]}]}