{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe NDC80 complex is a core, evolutionarily conserved kinetochore component that physically links chromosomes to dynamic spindle microtubules during mitosis. Composed of four subunits – including Hec1 (also known as NDC80), Nuf2, Spc24, and Spc25 – the complex forms an elongated “rod‐like” structure with distinct globular domains. These domains, notably the calponin‐homology regions and a positively charged, unstructured N‑terminal “tail” of Hec1, allow the complex to bind microtubules in a cooperative and electrostatically regulated manner. Structural and reconstitution studies demonstrate that the NDC80 complex contacts tubulin at both intra‐ and inter‐dimer interfaces, enabling it to stabilize both polymerizing and depolymerizing microtubule tips."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "7"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nThe microtubule‐binding activity of the NDC80 complex is finely tuned by multiple post‐translational modifications and protein–protein interactions. Several studies have shown that kinases such as Aurora B, Aurora A, and even Nek2 phosphorylate specific serine residues in the Hec1 N‑terminal tail (for example, S55, S69, and S165), thereby functioning as a “rheostat” that modulates the affinity of the complex for microtubules, promotes error correction, and regulates spindle checkpoint signaling. In addition, acetylation of Hec1 by TIP60 (and its reversal by SIRT1) further intersects with these phosphorylation events to control kinetochore–microtubule dynamics. The unstructured internal loop of Hec1 additionally serves as a platform for binding key regulatory factors—including Cdt1, the Ska complex, and Mps1—and even interacts with centrosomal proteins, which together ensure correct kinetochore assembly and the stabilization of end‐on microtubule attachments."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "8", "end_ref": "28"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAltered expression and mis‐regulation of the NDC80 complex have been linked to chromosomal instability and tumorigenesis. Overexpression of Hec1 and other complex subunits is observed in a variety of cancers—including those of the colon, ovary, liver, and stomach—and correlates with defective kinetochore–microtubule attachment, erroneous chromosome segregation, and an impaired spindle checkpoint. Such aberrations contribute to aneuploidy and may affect the sensitivity of tumor cells to antimitotic agents like paclitaxel. Furthermore, additional layers of regulation (for instance, via interactions with splicing factors or cell cycle regulators in the pRb pathway) suggest that precise control of NDC80 expression and activity is essential for maintaining genomic stability, making it a prospective diagnostic marker and therapeutic target in human cancer."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "29", "end_ref": "39"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jennifer G DeLuca, Yimin Dong, Polla Hergert, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e04-09-0852"}], "href": "https://doi.org/10.1091/mbc.e04-09-0852"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15548592"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15548592"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Claudio Ciferri, Jennifer De Luca, Silvia Monzani, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M504070200"}], "href": "https://doi.org/10.1074/jbc.M504070200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15961401"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15961401"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Jennifer G DeLuca, Walter E Gall, Claudio Ciferri, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Kinetochore microtubule dynamics and attachment stability are regulated by Hec1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2006.09.047"}], "href": "https://doi.org/10.1016/j.cell.2006.09.047"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17129782"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17129782"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Ronnie R Wei, Jawdat Al-Bassam, Stephen C Harrison "}, {"type": "b", "children": [{"type": "t", "text": "The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Struct Mol Biol (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nsmb1186"}], "href": "https://doi.org/10.1038/nsmb1186"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17195848"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17195848"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Claudio Ciferri, Sebastiano Pasqualato, Emanuela Screpanti, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2008.03.020"}], "href": "https://doi.org/10.1016/j.cell.2008.03.020"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18455984"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18455984"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Elizabeth M Wilson-Kubalek, Iain M Cheeseman, Craig Yoshioka, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Orientation and structure of the Ndc80 complex on the microtubule lattice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.200804170"}], "href": "https://doi.org/10.1083/jcb.200804170"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18794333"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18794333"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Gregory M Alushin, Vincent H Ramey, Sebastiano Pasqualato, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Ndc80 kinetochore complex forms oligomeric arrays along microtubules."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nature09423"}], "href": "https://doi.org/10.1038/nature09423"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20944740"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20944740"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Silvia Martin-Lluesma, Volker M Stucke, Erich A Nigg "}, {"type": "b", "children": [{"type": "t", "text": "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1075596"}], "href": "https://doi.org/10.1126/science.1075596"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12351790"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12351790"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Yumay Chen, Daniel J Riley, Lei Zheng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M207069200"}], "href": "https://doi.org/10.1074/jbc.M207069200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12386167"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12386167"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "J Du, X Cai, J Yao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/onc.2008.34"}], "href": "https://doi.org/10.1038/onc.2008.34"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18297113"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18297113"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Keith F DeLuca, Susanne M A Lens, Jennifer G DeLuca "}, {"type": "b", "children": [{"type": "t", "text": "Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.072629"}], "href": "https://doi.org/10.1242/jcs.072629"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21266467"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21266467"}]}, {"type": "r", "ref": 12, "children": [{"type": "t", "text": "Alexander Schleiffer, Michael Maier, Gabriele Litos, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CENP-T proteins are conserved centromere receptors of the Ndc80 complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb2493"}], "href": "https://doi.org/10.1038/ncb2493"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22561346"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22561346"}]}, {"type": "r", "ref": 13, "children": [{"type": "t", "text": "Soonjoung Kim, Hongtao Yu "}, {"type": "b", "children": [{"type": "t", "text": "Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.201407074"}], "href": "https://doi.org/10.1083/jcb.201407074"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25601404"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25601404"}]}, {"type": "r", "ref": 14, "children": [{"type": "t", "text": "Pim J Huis In 't Veld, Sadasivam Jeganathan, Arsen Petrovic, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Molecular basis of outer kinetochore assembly on CENP-T."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Elife (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7554/eLife.21007"}], "href": "https://doi.org/10.7554/eLife.21007"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28012276"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28012276"}]}, {"type": "r", "ref": 15, "children": [{"type": "t", "text": "Lynsie J R Sundin, Geoffrey J Guimaraes, Jennifer G Deluca "}, {"type": "b", "children": [{"type": "t", "text": "The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E10-08-0671"}], "href": "https://doi.org/10.1091/mbc.E10-08-0671"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21270439"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21270439"}]}, {"type": "r", "ref": 16, "children": [{"type": "t", "text": "Neil T Umbreit, Daniel R Gestaut, Jerry F Tien, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Ndc80 kinetochore complex directly modulates microtubule dynamics."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1209615109"}], "href": "https://doi.org/10.1073/pnas.1209615109"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22908300"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22908300"}]}, {"type": "r", "ref": 17, "children": [{"type": "t", "text": "Gregory M Alushin, Vivek Musinipally, Daniel Matson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Multimodal microtubule binding by the Ndc80 kinetochore complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Struct Mol Biol (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nsmb.2411"}], "href": "https://doi.org/10.1038/nsmb.2411"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23085714"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23085714"}]}, {"type": "r", "ref": 18, "children": [{"type": "t", "text": "Anatoly V Zaytsev, Jeanne E Mick, Evgeny Maslennikov, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Multisite phosphorylation of the NDC80 complex gradually tunes its microtubule-binding affinity."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E14-11-1539"}], "href": "https://doi.org/10.1091/mbc.E14-11-1539"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25808492"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25808492"}]}, {"type": "r", "ref": 19, "children": [{"type": "t", "text": "Dileep Varma, Srikripa Chandrasekaran, Lynsie J R Sundin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb2489"}], "href": "https://doi.org/10.1038/ncb2489"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22581055"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22581055"}]}, {"type": "r", "ref": 20, "children": [{"type": "t", "text": "Keith F DeLuca, Amanda Meppelink, Amanda J Broad, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.201707160"}], "href": "https://doi.org/10.1083/jcb.201707160"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29187526"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29187526"}]}, {"type": "r", "ref": 21, "children": [{"type": "t", "text": "Randy Wei, Bryan Ngo, Guikai Wu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E11-01-0012"}], "href": "https://doi.org/10.1091/mbc.E11-01-0012"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21832156"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21832156"}]}, {"type": "r", "ref": 22, "children": [{"type": "t", "text": "Gang Zhang, Christian D Kelstrup, Xiao-Wen Hu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.104208"}], "href": "https://doi.org/10.1242/jcs.104208"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22454517"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22454517"}]}, {"type": "r", "ref": 23, "children": [{"type": "t", "text": "Tongge Zhu, Zhen Dou, Bo Qin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Phosphorylation of microtubule-binding protein Hec1 by mitotic kinase Aurora B specifies spindle checkpoint kinase Mps1 signaling at the kinetochore."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M113.507970"}], "href": "https://doi.org/10.1074/jbc.M113.507970"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24187132"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24187132"}]}, {"type": "r", "ref": 24, "children": [{"type": "t", "text": "Shasha Hua, Zhikai Wang, Kai Jiang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CENP-U cooperates with Hec1 to orchestrate kinetochore-microtubule attachment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M110.174946"}], "href": "https://doi.org/10.1074/jbc.M110.174946"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21056971"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21056971"}]}, {"type": "r", "ref": 25, "children": [{"type": "t", "text": "John G Tooley, Stephanie A Miller, P Todd Stukenberg "}, {"type": "b", "children": [{"type": "t", "text": "The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E10-07-0626"}], "href": "https://doi.org/10.1091/mbc.E10-07-0626"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21325630"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21325630"}]}, {"type": "r", "ref": 26, "children": [{"type": "t", "text": "Geetika Sethi, Harsh B Pathak, Hong Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "An RNA interference lethality screen of the human druggable genome to identify molecular vulnerabilities in epithelial ovarian cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0047086"}], "href": "https://doi.org/10.1371/journal.pone.0047086"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23056589"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23056589"}]}, {"type": "r", "ref": 27, "children": [{"type": "t", "text": "Gangyin Zhao, Yubao Cheng, Ping Gui, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Dynamic acetylation of the kinetochore-associated protein HEC1 ensures accurate microtubule-kinetochore attachment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.RA118.003844"}], "href": "https://doi.org/10.1074/jbc.RA118.003844"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30409912"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30409912"}]}, {"type": "r", "ref": 28, "children": [{"type": "t", "text": "Alexandra F Long, Dylan B Udy, Sophie Dumont "}, {"type": "b", "children": [{"type": "t", "text": "Hec1 Tail Phosphorylation Differentially Regulates Mammalian Kinetochore Coupling to Polymerizing and Depolymerizing Microtubules."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Curr Biol (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cub.2017.04.058"}], "href": "https://doi.org/10.1016/j.cub.2017.04.058"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28552353"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28552353"}]}, {"type": "r", "ref": 29, "children": [{"type": "t", "text": "Gennadi V Glinsky, Olga Berezovska, Anna B Glinskii "}, {"type": "b", "children": [{"type": "t", "text": "Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI23412"}], "href": "https://doi.org/10.1172/JCI23412"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15931389"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15931389"}]}, {"type": "r", "ref": 30, "children": [{"type": "t", "text": "Naoyuki Kaneko, Koh Miura, Zhaodi Gu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2009.10.127"}], "href": "https://doi.org/10.1016/j.bbrc.2009.10.127"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19878654"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19878654"}]}, {"type": "r", "ref": 31, "children": [{"type": "t", "text": "Ivan Bièche, Sophie Vacher, François Lallemand, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cancer (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/1476-4598-10-23"}], "href": "https://doi.org/10.1186/1476-4598-10-23"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21352579"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21352579"}]}, {"type": "r", "ref": 32, "children": [{"type": "t", "text": "Lin-Ling Ju, Lin Chen, Jun-Hong Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Effect of NDC80 in human hepatocellular carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "World J Gastroenterol (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3748/wjg.v23.i20.3675"}], "href": "https://doi.org/10.3748/wjg.v23.i20.3675"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28611520"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28611520"}]}, {"type": "r", "ref": 33, "children": [{"type": "t", "text": "Tae Yeon Yoo, Jeong-Mo Choi, William Conway, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Elife (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7554/eLife.36392"}], "href": "https://doi.org/10.7554/eLife.36392"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30044223"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30044223"}]}, {"type": "r", "ref": 34, "children": [{"type": "t", "text": "B Xu, D-P Wu, R-T Xie, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Elevated NDC80 expression is associated with poor prognosis in osteosarcoma patients."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Eur Rev Med Pharmacol Sci (2017)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28537682"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28537682"}]}, {"type": "r", "ref": 35, "children": [{"type": "t", "text": "Guikai Wu, Randy Wei, Eric Cheng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e08-11-1123"}], "href": "https://doi.org/10.1091/mbc.e08-11-1123"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19776357"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19776357"}]}, {"type": "r", "ref": 36, "children": [{"type": "t", "text": "Qing-qing Mo, Ping-bo Chen, Xin Jin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to paclitaxel."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Acta Pharmacol Sin (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/aps.2012.197"}], "href": "https://doi.org/10.1038/aps.2012.197"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23474708"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23474708"}]}, {"type": "r", "ref": 37, "children": [{"type": "t", "text": "Chiara Ferretti, Pierangela Totta, Mario Fiore, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Expression of the kinetochore protein Hec1 during the cell cycle in normal and cancer cells and its regulation by the pRb pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Cycle (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4161/cc.9.20.13457"}], "href": "https://doi.org/10.4161/cc.9.20.13457"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20948316"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20948316"}]}, {"type": "r", "ref": 38, "children": [{"type": "t", "text": "Kenji Iemura, Toyoaki Natsume, Kayoko Maehara, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.202006116"}], "href": "https://doi.org/10.1083/jcb.202006116"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33988677"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33988677"}]}, {"type": "r", "ref": 39, "children": [{"type": "t", "text": "Claudia Pellacani, Elisabetta Bucciarelli, Fioranna Renda, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Elife (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7554/eLife.40325"}], "href": "https://doi.org/10.7554/eLife.40325"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30475206"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30475206"}]}]}]}