NFYB Gene

Name	nuclear transcription factor Y, beta
Description	The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nStudies on the NF‐Y complex have revealed that the NFYB subunit, together with NFYC, forms a histone‐fold dimer that specifically binds the CCAAT box—a ubiquitous element in eukaryotic promoters. Structural and modeling analyses show that NFYB, as an integral component of the NF‐Y trimer, contributes to DNA bending and nucleosome‐like binding, thereby facilitating the assembly of transcriptional machinery at target promoters. This unique configuration reinforces NFYB’s role in providing both sequence‐specific and nonspecific contacts that are central to gene activation and chromatin modification. (‎."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nBeyond its architectural function, NFYB is critical for the regulation of cell cycle and DNA repair genes. In promoters driving cell cycle progression (e.g., the cyclin B2 promoter), NF‐Y trimers containing NFYB bind multiple CCAAT boxes, often in cooperation with coactivators like p300, to fine‐tune transcriptional output. In addition, enhanced NFYB levels have been linked to transcriptional regulation events that lead to downregulation of key genes such as Top2α, thereby contributing to drug resistance in tumor cells. Moreover, NFYB participates in the activation of stress response pathways—as seen in its involvement with the IER5–p300 module regulating Cdc25B—and underpins the assembly of transcriptional programs that control checkpoint signaling. (‎."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "3", "end_ref": "6"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nNFYB also plays prominent roles in mediating cellular responses to chemotherapeutic agents. In colorectal cancer cells resistant to oxaliplatin, elevated NFYB drives the transcription of E2F1 and sustains CHK1 expression, thereby helping cancer cells evade apoptosis and maintain proliferative capacity. Complementarily, genome-wide analyses have identified NFYB as both a direct target of E2F1 and a pro-survival factor whose depletion enhances E2F1-induced apoptosis. In lymphoma, recruitment of NFYB by the long noncoding RNA H19 has been shown to upregulate MBTD1, promoting resistance to doxorubicin. Additionally, in glioblastoma, NFYB overexpression activates HDAC5, which in turn attenuates SHMT2 expression and glycolytic activity, ultimately increasing sensitivity to temozolomide. (‎."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "7", "end_ref": "11"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFurthermore, dynamic incorporation of NFYB at CCAAT-containing promoters is key to developmental gene regulation. In differentiating cells, fluctuations in NFYB complex binding correlate with the activation or repression of pivotal regulators such as the ID gene family, suggesting that NFYB is instrumental in modulating local chromatin environments and associated histone marks. Together, these findings underscore the multifaceted regulatory functions of NFYB in transcription, cellular homeostasis, and response to both developmental cues and chemotherapeutic stress. (‎."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Christophe Romier, Fabienne Cocchiarella, Roberto Mantovani, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M209635200"}], "href": "https://doi.org/10.1074/jbc.M209635200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12401788"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12401788"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Marco Nardini, Nerina Gnesutta, Giacomo Donati, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Sequence-specific transcription factor NF-Y displays histone-like DNA binding and H2B-like ubiquitination."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2012.11.047"}], "href": "https://doi.org/10.1016/j.cell.2012.11.047"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23332751"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23332751"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Joseph D Fleming, Giulio Pavesi, Paolo Benatti, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NF-Y coassociates with FOS at promoters, enhancers, repetitive elements, and inactive chromatin regions, and is stereo-positioned with growth-controlling transcription factors."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genome Res (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1101/gr.148080.112"}], "href": "https://doi.org/10.1101/gr.148080.112"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23595228"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23595228"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Valentina Salsi, Giuseppina Caretti, Mark Wasner, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M210065200"}], "href": "https://doi.org/10.1074/jbc.M210065200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12482752"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12482752"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Taido Matsui, Yuko Katsuno, Tomoharu Inoue, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Negative regulation of Chk2 expression by p53 is dependent on the CCAAT-binding transcription factor NF-Y."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M403232200"}], "href": "https://doi.org/10.1074/jbc.M403232200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15044452"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15044452"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Satoki Nakamura, Yasuyuki Nagata, Lin Tan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Transcriptional repression of Cdc25B by IER5 inhibits the proliferation of leukemic progenitor cells through NF-YB and p300 in acute myeloid leukemia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0028011"}], "href": "https://doi.org/10.1371/journal.pone.0028011"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22132193"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22132193"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Zejun Fang, Chaoju Gong, Songshan Yu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NFYB-induced high expression of E2F1 contributes to oxaliplatin resistance in colorectal cancer via the enhancement of CHK1 signaling."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Lett (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.canlet.2017.11.040"}], "href": "https://doi.org/10.1016/j.canlet.2017.11.040"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29203250"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29203250"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Xiaolei Jiang, Joseph Roy Nevins, Igor Shats, et al. "}, {"type": "b", "children": [{"type": "t", "text": "E2F1-Mediated Induction of NFYB Attenuates Apoptosis via Joint Regulation of a Pro-Survival Transcriptional Program."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0127951"}], "href": "https://doi.org/10.1371/journal.pone.0127951"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26039627"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26039627"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Farideh Moeinvaziri, Maryam Shahhoseini "}, {"type": "b", "children": [{"type": "t", "text": "Epigenetic role of CCAAT box-binding transcription factor NF-Y on ID gene family in human embryonic carcinoma cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "IUBMB Life (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/iub.1443"}], "href": "https://doi.org/10.1002/iub.1443"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26509926"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26509926"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Lili Feng, Qinying Yan, Xiaofang Xu "}, {"type": "b", "children": [{"type": "t", "text": "Long Non-coding RNA H19 Recruits NFYB to Activate MBTD1 and Regulate Doxorubicin Resistance in Lymphoma Cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biotechnol (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s12033-022-00600-2"}], "href": "https://doi.org/10.1007/s12033-022-00600-2"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36434485"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36434485"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Yingfan Zhang, Haoxuan Huang, Peikun Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NFYB increases chemosensitivity in glioblastoma by promoting HDAC5-mediated transcriptional inhibition of SHMT2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Neuropathol Exp Neurol (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/jnen/nlad073"}], "href": "https://doi.org/10.1093/jnen/nlad073"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37742129"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37742129"}]}]}]}
Synonyms	HAP3, NF-YB
Proteins	NFYB_HUMAN
NCBI Gene ID	4801
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

NFYB has 6,186 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 115 datasets.

Click the + buttons to view associations for NFYB from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

NFYB Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by NFYB gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of NFYB gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of NFYB gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of NFYB gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of NFYB gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of NFYB gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of NFYB gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	Biocarta Pathways	pathways involving NFYB protein from the Biocarta Pathways dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of NFYB gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of NFYB gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of NFYB gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of NFYB gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of NFYB gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of NFYB gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with NFYB protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of NFYB gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of NFYB gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of NFYB gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of NFYB gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing NFYB protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing NFYB protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing NFYB protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with NFYB protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with NFYB protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	CORUM Protein Complexes	protein complexs containing NFYB protein from the CORUM Protein Complexes dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with NFYB gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with NFYB gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with NFYB gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of NFYB protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with NFYB gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with NFYB gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with NFYB gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with NFYB gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at NFYB gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of NFYB gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of NFYB gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing NFYB from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of NFYB gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with NFYB gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing NFYB from the GeneSigDB Published Gene Signatures dataset.