{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nPKMYT1, also known as Myt1, is a membrane‐associated member of the Wee1 kinase family that plays a central role in cell cycle regulation. It phosphorylates CDK1 (Cdc2) on key residues (Thr14 and Tyr15) to prevent premature entry into mitosis, a process modulated by upstream kinases such as Plk1 and MEK1. In addition to this canonical function, PKMYT1 has been implicated in controlling the reassembly of intracellular membrane structures during telophase, thereby ensuring proper Golgi and endoplasmic reticulum organization and maintenance of cellular architecture."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "5"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn multiple cancer types—including colorectal, prostate, hepatocellular, renal, lung, and triple‐negative breast cancers—PKMYT1 is frequently overexpressed and exerts oncogenic functions. For example, in CCNE1‐amplified tumors, inhibition of PKMYT1 triggers unscheduled CDK1 activation resulting in aberrant mitosis and cell death, highlighting its potential as a therapeutic vulnerability. In other contexts, elevated PKMYT1 activity supports cell proliferation, migration, and invasion by modulating key signaling pathways such as beta‐catenin/TCF and Notch, contributing to epithelial–mesenchymal transition and drug resistance. Moreover, studies in various models have demonstrated that PKMYT1 not only enhances tumor cell growth but also mediates resistance to Wee1 inhibitors, underscoring its importance in maintaining the G2 checkpoint and tumor cell survival."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "6", "end_ref": "16"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAdditional investigations have extended the functional repertoire of PKMYT1. In virus‐infected cells, for instance, inhibition of PKMYT1 markedly reduces the efficiency of herpesvirus reactivation, suggesting its role in cell cycle environments that favor lytic infection. Similarly, studies in ovarian and clear cell renal cell carcinomas further demonstrate that high PKMYT1 expression correlates with advanced tumor grade, poor overall survival, and enhanced malignant progression, making it a promising prognostic biomarker and therapeutic target across diverse malignancies."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "17", "end_ref": "19"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Hiroyuki Nakajima, Fumiko Toyoshima-Morimoto, Eri Taniguchi, et al. 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