RASSF8 Gene

Name	Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Description	This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRASSF8 is a conserved member of the RAS‐association domain family that functions predominantly as a tumor suppressor. Studies in lung cancer have shown that RASSF8 transcript levels are markedly reduced in adenocarcinomas compared with normal lung tissue, and its expression suppresses anchorage‐independent growth and clonogenic activity (for example, in lung adenocarcinoma studies."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMechanistically, RASSF8 localizes both to the nucleus and to the plasma membrane at sites of cell–cell adhesion where it interacts with key adherens junction components such as E‐cadherin and β‐catenin. Loss of RASSF8 destabilizes these junctions, promoting nuclear translocation of β‐catenin and NF‐κB (p65) signaling, which in turn enhances cell migration and tumor cell invasion. In cervical cancer cells, for instance, overexpression of miR-224 directly targets RASSF8, thereby diminishing its protective role and facilitating tumor progression. Similar observations have been made in esophageal squamous cell carcinoma and melanoma where reduced RASSF8 levels associate with enhanced migratory capabilities and aggressive behavior."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "4", "end_ref": "6"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAdditional layers of regulation contribute to the loss of RASSF8 function in cancer. In breast cancer, transcriptional repression by E4BP4 in concert with histone methyltransferases has been identified as a mechanism for RASSF8 downregulation, highlighting the role of epigenetic modulation in its expression. In epithelial ovarian cancer, a circular RNA (circCERS6) acts as a molecular sponge for miR-630, resulting in the upregulation of RASSF8 and subsequent inhibition of cell proliferation, migration, invasion, and epithelial–mesenchymal transition. These studies underscore the multifaceted regulatory networks that affect RASSF8 levels and illustrate its critical role in maintaining cellular homeostasis and suppressing tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "F S Falvella, G Manenti, M Spinola, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of RASSF8 as a candidate lung tumor suppressor gene."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/sj.onc.1209422"}], "href": "https://doi.org/10.1038/sj.onc.1209422"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16462760"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16462760"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "F Stefania Falvella, Monica Spinola, Giacomo Manenti, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Common polymorphisms in D12S1034 flanking genes RASSF8 and BHLHB3 are not associated with lung adenocarcinoma risk."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Lung Cancer (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.lungcan.2006.11.008"}], "href": "https://doi.org/10.1016/j.lungcan.2006.11.008"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17194498"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17194498"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "F E Lock, N Underhill-Day, T Dunwell, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The RASSF8 candidate tumor suppressor inhibits cell growth and regulates the Wnt and NF-kappaB signaling pathways."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/onc.2010.192"}], "href": "https://doi.org/10.1038/onc.2010.192"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20514026"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20514026"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Jinhua Wang, Wei Hua, Sharon K Huang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RASSF8 regulates progression of cutaneous melanoma through nuclear factor-κb."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.5030"}], "href": "https://doi.org/10.18632/oncotarget.5030"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26334503"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26334503"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Lan Zhang, Jian-Hua Wang, Rong-Xin Liang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RASSF8 downregulation promotes lymphangiogenesis and metastasis in esophageal squamous cell carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.5923"}], "href": "https://doi.org/10.18632/oncotarget.5923"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26439687"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26439687"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "YongJie Huang, Yang Li, Fen F Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Over-Expressed miR-224 Promotes the Progression of Cervical Cancer via Targeting RASSF8."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0162378"}], "href": "https://doi.org/10.1371/journal.pone.0162378"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27626930"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27626930"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Isai Pratha Karthik, Pavitra Desai, Sudarkodi Sukumar, et al. "}, {"type": "b", "children": [{"type": "t", "text": "E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.RA117.000623"}], "href": "https://doi.org/10.1074/jbc.RA117.000623"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29467226"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29467226"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Xiaoqin Li, Xuemei Jiang, Jing Lu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CircCERS6 Suppresses the Development of Epithelial Ovarian Cancer Through Mediating miR-630/RASSF8."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Genet (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s10528-022-10227-2"}], "href": "https://doi.org/10.1007/s10528-022-10227-2"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35676548"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35676548"}]}]}]}
Synonyms	C12orf2, HOJ1
Proteins	RASF8_HUMAN
NCBI Gene ID	11228
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

RASSF8 has 7,573 functional associations with biological entities spanning 9 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 107 datasets.

Click the + buttons to view associations for RASSF8 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

RASSF8 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of RASSF8 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of RASSF8 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of RASSF8 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of RASSF8 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of RASSF8 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of RASSF8 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of RASSF8 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of RASSF8 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of RASSF8 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of RASSF8 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of RASSF8 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of RASSF8 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with RASSF8 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with RASSF8 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of RASSF8 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of RASSF8 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of RASSF8 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of RASSF8 gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing RASSF8 protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of RASSF8 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing RASSF8 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing RASSF8 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with RASSF8 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with RASSF8 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of RASSF8 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with RASSF8 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with RASSF8 gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with RASSF8 gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of RASSF8 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by RASSF8 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores	diseases associated with RASSF8 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with RASSF8 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with RASSF8 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with RASSF8 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with RASSF8 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with RASSF8 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at RASSF8 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of RASSF8 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of RASSF8 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing RASSF8 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.