RNPS1 Gene

HGNC Family	RNA binding motif containing (RBM)
Name	RNA binding protein S1, serine-rich domain
Description	This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRNPS1 is a multifaceted RNA‐binding protein that plays essential roles in pre‐mRNA processing and mRNA quality control. In one major function, RNPS1 is a component of the postsplicing complex that is deposited upstream of exon–exon junctions, where it directly interacts with the conserved Upf complex to trigger nonsense-mediated decay (NMD) when tethered to the 3′ untranslated region. In addition, RNPS1 acts as a potent splicing activator that incorporates into active spliceosomes, promoting both the formation of ATP-dependent complexes and efficient generation of spliced products—a function that is intricately modulated by its phosphorylation (notably at serine 53 by casein kinase II)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nBeyond its roles in splicing and mRNA surveillance, RNPS1 helps maintain genomic stability. It collaborates with the splicing regulator ASF/SF2 to form ribonucleoprotein complexes on newly synthesized transcripts, thereby preventing the formation of deleterious transcription-induced R-loops that can lead to DNA fragmentation and hypermutation. Modulation of RNPS1’s function is further achieved through regulation of its ubiquitination status; USP4 specifically deubiquitinates RNPS1 by removing K63-linked polyubiquitin chains—a process that may fine-tune its activity in RNA processing."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nRNPS1 has also emerged as a key player in cancer biology. In various tumor types, including cervical carcinoma, head and neck squamous cell carcinoma, and uterine corpus endometrial carcinoma, elevated expression of RNPS1 correlates with increased cell proliferation, enhanced colony formation, and greater migratory and invasive capacities. Mechanistically, RNPS1 has been shown to regulate alternative splicing events that favor the production of tumor-associated gene isoforms (for example, the oncogenic splice variant Rac1b and altered RhoA isoforms) and to stabilize proteins such as NAT10 by preventing their ubiquitin-mediated degradation. Together, these activities contribute to a cancer cell phenotype marked by increased chemoresistance and enhanced malignant progression."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "5", "end_ref": "7"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "J Lykke-Andersen, M D Shu, J A Steitz "}, {"type": "b", "children": [{"type": "t", "text": "Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2001)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1062786"}], "href": "https://doi.org/10.1126/science.1062786"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11546874"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11546874"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Janeen H Trembley, Sawako Tatsumi, Eiji Sakashita, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Activation of pre-mRNA splicing by human RNPS1 is regulated by CK2 phosphorylation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biol (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/MCB.25.4.1446-1457.2005"}], "href": "https://doi.org/10.1128/MCB.25.4.1446-1457.2005"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15684395"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15684395"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Xialu Li, Tianhui Niu, James L Manley "}, {"type": "b", "children": [{"type": "t", "text": "The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "RNA (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1261/rna.734407"}], "href": "https://doi.org/10.1261/rna.734407"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17959926"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17959926"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Seul-Ki Kwon, Eun-Hea Kim, Kwang-Hyun Baek "}, {"type": "b", "children": [{"type": "t", "text": "RNPS1 is modulated by ubiquitin-specific protease 4."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "FEBS Lett (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/1873-3468.12531"}], "href": "https://doi.org/10.1002/1873-3468.12531"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27990632"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27990632"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Xiaojuan Liu, Hui Ma, Lisha Ma, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RNA-binding protein with serine-rich domain 1 regulates microsatellite instability of uterine corpus endometrial adenocarcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Clinics (Sao Paulo) (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.6061/clinics/2021/e3318"}], "href": "https://doi.org/10.6061/clinics/2021/e3318"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34817046"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34817046"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Bhagyashree Deka, Pratap Chandra, Priyanka Yadav, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RNPS1 functions as an oncogenic splicing factor in cervical cancer cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "IUBMB Life (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/iub.2686"}], "href": "https://doi.org/10.1002/iub.2686"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36300671"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36300671"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Xiaochen Wang, Rongsong Ling, Yurong Peng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac"}, {"type": "a", "children": [{"type": "t", "text": "sup"}], "href": "sup"}, {"type": "t", "text": "4"}, {"type": "a", "children": [{"type": "t", "text": "/sup"}], "href": "/sup"}, {"type": "t", "text": "C modification."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Oral Sci (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41368-023-00276-7"}], "href": "https://doi.org/10.1038/s41368-023-00276-7"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38246918"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38246918"}]}]}]}
Synonyms	E5.1
Proteins	RNPS1_HUMAN
NCBI Gene ID	10921
API
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

RNPS1 has 8,449 functional associations with biological entities spanning 9 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 116 datasets.

Click the + buttons to view associations for RNPS1 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

RNPS1 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by RNPS1 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of RNPS1 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of RNPS1 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of RNPS1 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of RNPS1 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of RNPS1 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of RNPS1 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of RNPS1 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of RNPS1 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of RNPS1 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of RNPS1 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with RNPS1 protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of RNPS1 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of RNPS1 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of RNPS1 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of RNPS1 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing RNPS1 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing RNPS1 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with RNPS1 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with RNPS1 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	CORUM Protein Complexes	protein complexs containing RNPS1 protein from the CORUM Protein Complexes dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of RNPS1 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with RNPS1 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with RNPS1 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with RNPS1 gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with RNPS1 gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of RNPS1 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with RNPS1 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with RNPS1 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with RNPS1 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with RNPS1 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with RNPS1 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at RNPS1 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of RNPS1 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of RNPS1 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing RNPS1 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with RNPS1 gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GAD High Level Gene-Disease Associations	diseases associated with RNPS1 gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of RNPS1 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with RNPS1 gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.