STRADA Gene

Name	STE20-related kinase adaptor alpha
Description	The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nSTRADA, a pseudokinase and essential adaptor for the tumor suppressor LKB1, plays a central role in modulating LKB1 activity and localization. Deacetylation of LKB1 (via SIRT1) increases its association with STRADA, thereby enhancing LKB1’s kinase activity and promoting its exit from the nucleus to the cytoplasm. Structural and biochemical studies reveal that STRADA, despite lacking catalytic function, adopts a closed “active‐like” conformation that acts as a pseudosubstrate for LKB1 and, in conjunction with the scaffolding protein MO25, stabilizes its active state. In epithelial cells, the STRADA–LKB1–MO25 complex is critical for establishing cell polarity, and STRADA further directs nucleocytoplasmic shuttling of LKB1 by linking it to nuclear export machinery."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "6"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nDefects in STRADA function have profound developmental and pathological consequences. Homozygous deletions or point mutations in STRADA give rise to neurodevelopmental disorders such as polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. In these conditions, the inability of STRADA to export LKB1 from the nucleus leads to its aberrant accumulation, triggering dysregulated mTORC1 signaling and cortical malformations. These findings underscore STRADA’s critical contribution to brain development as well as its role in moderating LKB1-dependent cell cycle and metabolic pathways."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "7", "end_ref": "9"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nRecent studies further highlight the diversity and therapeutic potential of STRADA. Novel splice isoforms of STRADA have been discovered in various tissues, suggesting differential effects on LKB1 complex assembly and downstream AMPK pathway activation. Moreover, structural models indicate that STRADA—even as a pseudokinase—can adopt distinct conformations modulated by ATP and MO25 binding. This conformational plasticity opens the door to the development of conformation‐specific inhibitors (similar to type II kinase inhibitors), proposing new avenues for pharmacological intervention in diseases linked to LKB1 signaling."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Fan Lan, Jose M Cacicedo, Neil Ruderman, et al. "}, {"type": "b", "children": [{"type": "t", "text": "SIRT1 modulation of the acetylation status, cytosolic localization, and activity of LKB1. Possible role in AMP-activated protein kinase activation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M805711200"}], "href": "https://doi.org/10.1074/jbc.M805711200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18687677"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18687677"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "A F Baas, J Boudeau, G P Sapkota, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO J (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/emboj/cdg292"}], "href": "https://doi.org/10.1093/emboj/cdg292"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12805220"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12805220"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Elton Zeqiraj, Beatrice Maria Filippi, Maria Deak, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Structure of the LKB1-STRAD-MO25 complex reveals an allosteric mechanism of kinase activation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Science (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/science.1178377"}], "href": "https://doi.org/10.1126/science.1178377"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19892943"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19892943"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Jean Paul ten Klooster, Marnix Jansen, Jin Yuan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25 polarization complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Dev Cell (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.devcel.2009.01.016"}], "href": "https://doi.org/10.1016/j.devcel.2009.01.016"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19386264"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19386264"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Elton Zeqiraj, Beatrice Maria Filippi, Simon Goldie, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ATP and MO25alpha regulate the conformational state of the STRADalpha pseudokinase and activation of the LKB1 tumour suppressor."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Biol (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pbio.1000126"}], "href": "https://doi.org/10.1371/journal.pbio.1000126"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19513107"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19513107"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Julia Dorfman, Ian G Macara "}, {"type": "b", "children": [{"type": "t", "text": "STRADalpha regulates LKB1 localization by blocking access to importin-alpha, and by association with Crm1 and exportin-7."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.e07-05-0454"}], "href": "https://doi.org/10.1091/mbc.e07-05-0454"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18256292"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18256292"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Ksenia A Orlova, Whitney E Parker, Gregory G Heuer, et al. "}, {"type": "b", "children": [{"type": "t", "text": "STRADalpha deficiency results in aberrant mTORC1 signaling during corticogenesis in humans and mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI41592"}], "href": "https://doi.org/10.1172/JCI41592"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20424326"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20424326"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Sarah Fogarty, D Grahame Hardie "}, {"type": "b", "children": [{"type": "t", "text": "C-terminal phosphorylation of LKB1 is not required for regulation of AMP-activated protein kinase, BRSK1, BRSK2, or cell cycle arrest."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M806152200"}], "href": "https://doi.org/10.1074/jbc.M806152200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18854318"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18854318"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Weimin Bi, Ian A Glass, Donna M Muzny, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Whole exome sequencing identifies the first STRADA point mutation in a patient with polyhydramnios, megalencephaly, and symptomatic epilepsy syndrome (PMSE)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Am J Med Genet A (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/ajmg.a.37727"}], "href": "https://doi.org/10.1002/ajmg.a.37727"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27170158"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27170158"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "P A Marignani, K D Scott, R Bagnulo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Novel splice isoforms of STRADalpha differentially affect LKB1 activity, complex assembly and subcellular localization."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Biol Ther (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4161/cbt.6.10.4787"}], "href": "https://doi.org/10.4161/cbt.6.10.4787"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17921699"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17921699"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Ryan H B Smith, Zaigham M Khan, Peter Man-Un Ung, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Type II Binders Targeting the \"GLR-Out\" Conformation of the Pseudokinase STRADα."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochemistry (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1021/acs.biochem.0c00714"}], "href": "https://doi.org/10.1021/acs.biochem.0c00714"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33440120"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33440120"}]}]}]}
Synonyms	LYK5, PMSE, STRAD, STRAD alpha, NY-BR-96, STLK
Proteins	STRAA_HUMAN
NCBI Gene ID	92335
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

STRADA has 5,284 functional associations with biological entities spanning 9 categories (molecular profile, organism, functional term, phrase or reference, disease, phenotype or trait, chemical, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 110 datasets.

Click the + buttons to view associations for STRADA from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

STRADA Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by STRADA gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of STRADA gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of STRADA gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of STRADA gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of STRADA gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of STRADA gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of STRADA gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of STRADA gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of STRADA gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of STRADA gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Gene Mutation Profiles	cell lines with STRADA gene mutations from the CCLE Cell Line Gene Mutation Profiles dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of STRADA gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of STRADA gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of STRADA gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing STRADA protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with STRADA protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with STRADA protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of STRADA gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with STRADA gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with STRADA gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of STRADA protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by STRADA gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with STRADA gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with STRADA gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with STRADA gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with STRADA gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at STRADA gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of STRADA gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of STRADA gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing STRADA from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with STRADA gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GAD High Level Gene-Disease Associations	diseases associated with STRADA gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of STRADA gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with STRADA gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing STRADA from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of STRADA gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.
	GEO Signatures of Differentially Expressed Genes for Gene Perturbations	gene perturbations changing expression of STRADA gene from the GEO Signatures of Differentially Expressed Genes for Gene Perturbations dataset.
	GEO Signatures of Differentially Expressed Genes for Kinase Perturbations	kinase perturbations changing expression of STRADA gene from the GEO Signatures of Differentially Expressed Genes for Kinase Perturbations dataset.
	GEO Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of STRADA gene from the GEO Signatures of Differentially Expressed Genes for Small Molecules dataset.
	GEO Signatures of Differentially Expressed Genes for Transcription Factor Perturbations	transcription factor perturbations changing expression of STRADA gene from the GEO Signatures of Differentially Expressed Genes for Transcription Factor Perturbations dataset.