STX5 Gene

HGNC Family	Syntaxins (STX)
Name	syntaxin 5
Description	This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nSyntaxin 5 (STX5) is a key t‐SNARE protein that orchestrates multiple steps of intracellular membrane trafficking, thereby ensuring proper Golgi integrity and function. Studies have shown that reduced STX5 expression results in Golgi fragmentation, underlining its essential role in maintaining Golgi architecture (via PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In addition, STX5 is a critical component of the SNARE machinery required for retrograde transport routes, as evidenced by its participation in the transport of both endogenous cargo (such as the mannose 6‐phosphate receptor) and exogenous toxins like Shiga and cholera toxins (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " Its interaction with the golgin tether GM130 links membrane tethering to fusion processes at early secretory sites (PMID"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ", while binding of the SM protein Sly1 to STX5 further facilitates SNARE complex assembly crucial for intra-Golgi transport (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Moreover, STX5 functions in autophagy by regulating later stages of the pathway via its involvement in ER-to-Golgi transport necessary for lysosomal enzyme activity (PMID"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ", and plays a central role in viral trafficking, as inhibition of STX5 markedly decreases adeno-associated virus serotype 2 (AAV2) delivery to the Golgi (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Furthermore, posttranslational modification of STX5, such as monoubiquitination during early mitosis, disrupts SNARE complex formation and orchestrates Golgi disassembly and subsequent reassembly (PMID"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": ", and loss of its short isoform via an alternative translation initiation event has been linked to severe multisystem disease characterized by improper glycosylation, thereby underlining its indispensable role in intra-Golgi transport (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nSTX5 also fulfills specialized functions beyond its classical role in vesicular transport. Notably, elevated levels of autoantibodies against STX5 have been identified as a potential serological marker for endometriosis (PMID"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": ", and direct interaction of STX5 with the very low density lipoprotein (VLDL) receptor suggests that it modulates receptor glycosylation and trafficking via a possible Golgi-bypass pathway (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": " In addition, binding of STX5 by the Golgin45 tether appears critical for restraining intercisternal fusion within the Golgi stack, thereby preserving Golgi architecture (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": " In endothelial cells, STX5 regulates the biogenesis and elongation of Weibel–Palade bodies, which is essential for proper von Willebrand factor storage and secretion (PMID"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "12"}]}, {"type": "t", "text": ", while in activated macrophages, its novel RNA-binding capacity facilitates the export of microRNAs, thus promoting inflammatory cytokine expression (PMID."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "13"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Kei Suga, Hiroshi Hattori, Ayako Saito, et al. "}, {"type": "b", "children": [{"type": "t", "text": "RNA interference-mediated silencing of the syntaxin 5 gene induces Golgi fragmentation but capable of transporting vesicles."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "FEBS Lett (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.febslet.2005.06.053"}], "href": "https://doi.org/10.1016/j.febslet.2005.06.053"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16081076"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16081076"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Mohamed Amessou, Alexandre Fradagrada, Thomas Falguières, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Syntaxin 16 and syntaxin 5 are required for efficient retrograde transport of several exogenous and endogenous cargo proteins."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.03436"}], "href": "https://doi.org/10.1242/jcs.03436"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17389686"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17389686"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Aipo Diao, Laura Frost, Yuichi Morohashi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M708401200"}], "href": "https://doi.org/10.1074/jbc.M708401200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18167358"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18167358"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Orly Laufman, Amir Kedan, Wanjin Hong, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO J (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/emboj.2009.168"}], "href": "https://doi.org/10.1038/emboj.2009.168"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19536132"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19536132"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Maurizio Renna, Catherine Schaffner, Ashley R Winslow, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.076489"}], "href": "https://doi.org/10.1242/jcs.076489"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21242315"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21242315"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Mathieu E Nonnenmacher, Jean-Christophe Cintrat, Daniel Gillet, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Syntaxin 5-dependent retrograde transport to the trans-Golgi network is required for adeno-associated virus transduction."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Virol (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/JVI.02520-14"}], "href": "https://doi.org/10.1128/JVI.02520-14"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25410859"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25410859"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Shijiao Huang, Danming Tang, Yanzhuang Wang "}, {"type": "b", "children": [{"type": "t", "text": "Monoubiquitination of Syntaxin 5 Regulates Golgi Membrane Dynamics during the Cell Cycle."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Dev Cell (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.devcel.2016.06.001"}], "href": "https://doi.org/10.1016/j.devcel.2016.06.001"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27404360"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27404360"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Peter T A Linders, Eveline C F Gerretsen, Angel Ashikov, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-021-26534-y"}], "href": "https://doi.org/10.1038/s41467-021-26534-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34711829"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34711829"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Motowo Nabeta, Yasuhito Abe, Yuki Takaoka, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of anti-syntaxin 5 autoantibody as a novel serum marker of endometriosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Reprod Immunol (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jri.2011.04.012"}], "href": "https://doi.org/10.1016/j.jri.2011.04.012"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21715015"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21715015"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Timo Wagner, Marco Dieckmann, Sebastian Jaeger, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Stx5 is a novel interactor of VLDL-R to affect its intracellular trafficking and processing."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Exp Cell Res (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.yexcr.2013.05.010"}], "href": "https://doi.org/10.1016/j.yexcr.2013.05.010"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23701949"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23701949"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Neeraj Tiwari, Morven Graham, Xinran Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-019-48875-x"}], "href": "https://doi.org/10.1038/s41598-019-48875-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31462665"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31462665"}]}, {"type": "r", "ref": 12, "children": [{"type": "t", "text": "Marije Kat, Ellie Karampini, Arie J Hoogendijk, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Syntaxin 5 determines Weibel-Palade body size and von Willebrand factor secretion by controlling Golgi architecture."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Haematologica (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3324/haematol.2021.280121"}], "href": "https://doi.org/10.3324/haematol.2021.280121"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35081689"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35081689"}]}, {"type": "r", "ref": 13, "children": [{"type": "t", "text": "Sourav Hom Choudhury, Shreya Bhattacharjee, Kamalika Mukherjee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Human antigen R transfers miRNA to Syntaxin 5 to synergize miRNA export from activated macrophages."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jbc.2024.107170"}], "href": "https://doi.org/10.1016/j.jbc.2024.107170"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38492777"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38492777"}]}]}]}
Synonyms	STX5A, SED5
Proteins	STX5_HUMAN
NCBI Gene ID	6811
API
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

STX5 has 6,672 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 113 datasets.

Click the + buttons to view associations for STX5 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

STX5 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by STX5 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of STX5 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of STX5 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of STX5 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of STX5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of STX5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of STX5 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of STX5 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of STX5 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of STX5 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of STX5 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with STX5 protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of STX5 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of STX5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of STX5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing STX5 protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of STX5 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing STX5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing STX5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing STX5 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing STX5 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with STX5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with STX5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with STX5 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with STX5 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with STX5 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of STX5 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DISEASES Curated Gene-Disease Association Evidence Scores 2025	diseases involving STX5 gene from the DISEASES Curated Gene-Disease Association Evidence Scores 2025 dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with STX5 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with STX5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with STX5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with STX5 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at STX5 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of STX5 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of STX5 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing STX5 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of STX5 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with STX5 gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing STX5 from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of STX5 gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.