{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nAlthough the request mentions TEX13D, none of the provided abstracts discuss TEX13D. Instead, these studies focus on DNA repair enzymes that counteract alkylation‐ and inflammation‐induced DNA damage. In one study, primary mouse embryonic fibroblasts lacking either Alkbh2 or Alkbh3 were shown to be approximately twofold more sensitive to the methylating agent MMS. In these cells, although both enzymes protect against MMS-induced cytotoxicity, only the deficiency of Alkbh2 resulted in a significant increase in mutation frequency, highlighting Alkbh2’s distinct role in preserving DNA fidelity following alkylation damage."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAnother study used chemically induced models of colitis and colon cancer in mice to reveal that inflammation-associated DNA lesions—specifically etheno-adducts induced by reactive oxygen and nitrogen species—are efficiently repaired not only by the alkyl adenine DNA glycosylase (AAG) but also by Alkbh2 and Alkbh3. Notably, the deficiency of all three repair enzymes resulted in a massively synergistic phenotype, underscoring their collective importance in protecting against inflammation-mediated carcinogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nA further investigation focused on the repair of specific alkylated thymidine lesions. Using nonreplicative double-stranded vectors containing site-specific lesions, the authors demonstrated that among several types of lesions examined (including N3-ethylthymidine), only N3-EtdT was efficiently repaired by both Alkbh2 and Alkbh3. This study was further supported by in vitro evidence showing the direct reversal of N3-EtdT by purified human Alkbh2, thereby providing key insights into the substrate specificity of these DNA repair enzymes in mammalian cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Stephanie L Nay, Dong-Hyun Lee, Steven E Bates, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Alkbh2 protects against lethality and mutation in primary mouse embryonic fibroblasts."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "DNA Repair (Amst) (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.dnarep.2012.02.005"}], "href": "https://doi.org/10.1016/j.dnarep.2012.02.005"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22429847"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22429847"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jennifer A Calvo, Lisiane B Meira, Chun-Yue I Lee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "DNA repair is indispensable for survival after acute inflammation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI63338"}], "href": "https://doi.org/10.1172/JCI63338"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22684101"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22684101"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Changjun You, Pengcheng Wang, Stephanie L Nay, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "ACS Chem Biol (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1021/acschembio.6b00085"}], "href": "https://doi.org/10.1021/acschembio.6b00085"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26930515"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26930515"}]}]}]}