{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe collection of abstracts provided focuses extensively on the biological activities of myosin light chain kinases (MLCK) in diverse tissues—from skeletal and cardiac muscle to epithelial and endothelial cells—but does not mention or address any function related to TRIM77. In skeletal muscle, for example, a dedicated MLCK isoform is essential for phosphorylating the myosin regulatory light chain (RLC) to potentiate force output during repetitive stimulation, as demonstrated by the loss of posttetanic potentiation when skeletal muscle MLCK is ablated (see."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Similarly, in cardiac muscle a distinct, Ca(2+)/calmodulin-activated cardiac MLCK (cMLCK) maintains basal RLC phosphorylation and proper contractile function; when cMLCK expression is reduced or eliminated, the hearts develop hypertrophy, sarcomere disorganization, and contractile failure (see."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "2", "end_ref": "5"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nOther studies in the set address MLCK’s roles in non‐muscle cells. In intestinal and endothelial epithelia, activation of MLCK by proinflammatory signals such as interleukin‐1β or tumor necrosis factor leads to phosphorylation events that disrupt tight junction integrity and promote increased permeability; these events are critical in models of intestinal inflammation and barrier dysfunction (see."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "6", "end_ref": "10"}]}, {"type": "t", "text": " In addition, other non‐muscle contexts—such as the regulation of nonmuscle myosin II filament assembly and cell motility or the modulation of insulin‐stimulated glucose transport in adipocytes—also highlight how MLCK activity, acting via Ca(2+)/calmodulin and upstream kinases, orchestrates cytoskeletal reorganization and cellular function (see."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, these studies reveal that MLCK isoforms serve as central mediators linking intracellular signaling (through Ca(2+)/calmodulin, MAPK, and Rho kinase pathways) to myosin regulatory light chain phosphorylation. This phosphorylation, in turn, regulates the mechanical and contractile properties of cells in a tissue-specific manner, whether coordinating muscle contraction, preserving epithelial barrier function, or modulating cell migration and metabolic processes (see."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "13", "end_ref": "26"}]}, {"type": "t", "text": " \nNotably, however, none of these reports mention or examine TRIM77. As a result, while the function of multiple MLCK isoforms is well documented in these abstracts, no information is provided regarding any role for TRIM77.\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Gang Zhi, Jeffrey W Ryder, Jian Huang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Myosin light chain kinase and myosin phosphorylation effect frequency-dependent potentiation of skeletal muscle contraction."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.0506846102"}], "href": "https://doi.org/10.1073/pnas.0506846102"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16299103"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16299103"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jason Y Chan, Morihiko Takeda, Laura E Briggs, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of cardiac-specific myosin light chain kinase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Circ Res (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1161/CIRCRESAHA.107.161687"}], "href": "https://doi.org/10.1161/CIRCRESAHA.107.161687"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18202317"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18202317"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Peiguo Ding, Jian Huang, Pavan K Battiprolu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Cardiac myosin light chain kinase is necessary for myosin regulatory light chain phosphorylation and cardiac performance in vivo."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M110.160499"}], "href": "https://doi.org/10.1074/jbc.M110.160499"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20943660"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20943660"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Michael T Massengill, Hassan M Ashraf, Rajib R Chowdhury, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Acute heart failure with cardiomyocyte atrophy induced in adult mice by ablation of cardiac myosin light chain kinase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cardiovasc Res (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/cvr/cvw069"}], "href": "https://doi.org/10.1093/cvr/cvw069"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27025239"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27025239"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Tatsuro Hitsumoto, Osamu Tsukamoto, Ken Matsuoka, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Circulation (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1161/CIRCULATIONAHA.122.062885"}], "href": "https://doi.org/10.1161/CIRCULATIONAHA.122.062885"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37128901"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37128901"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Rana Al-Sadi, Shuhong Guo, Dongmei Ye, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Mechanism of IL-1β modulation of intestinal epithelial barrier involves p38 kinase and activating transcription factor-2 activation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Immunol (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4049/jimmunol.1201876"}], "href": "https://doi.org/10.4049/jimmunol.1201876"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23656735"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23656735"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Chuanli Chen, Pei Wang, Qin Su, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Myosin light chain kinase mediates intestinal barrier disruption following burn injury."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0034946"}], "href": "https://doi.org/10.1371/journal.pone.0034946"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22529961"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22529961"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Masahiro Suzuki, Takashi Nagaishi, Motomi Yamazaki, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0088369"}], "href": "https://doi.org/10.1371/journal.pone.0088369"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24520376"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24520376"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Li-Ling Wu, Wei-Hao Peng, Wei-Ting Kuo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Am J Pathol (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.ajpath.2014.05.003"}], "href": "https://doi.org/10.1016/j.ajpath.2014.05.003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24911373"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24911373"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Fabeha Fazal, Kaiser M Bijli, Matthew Murrill, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Critical role of non-muscle myosin light chain kinase in thrombin-induced endothelial cell inflammation and lung PMN infiltration."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0059965"}], "href": "https://doi.org/10.1371/journal.pone.0059965"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23555849"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23555849"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Jordan R Beach, Kyle S Bruun, Lin Shao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb3463"}], "href": "https://doi.org/10.1038/ncb3463"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28114272"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28114272"}]}, {"type": "r", "ref": 12, "children": [{"type": "t", "text": "Xiaonan Han, Elizabeth Mann, Shila Gilbert, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Loss of guanylyl cyclase C (GCC) signaling leads to dysfunctional intestinal barrier."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0016139"}], "href": "https://doi.org/10.1371/journal.pone.0016139"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21305056"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21305056"}]}, {"type": "r", "ref": 13, "children": [{"type": "t", "text": "Manqi Deng, Carmen J Williams, Richard M Schultz "}, {"type": "b", "children": [{"type": "t", "text": "Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Dev Biol (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.ydbio.2004.11.013"}], "href": "https://doi.org/10.1016/j.ydbio.2004.11.013"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15680356"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15680356"}]}, {"type": "r", "ref": 14, "children": [{"type": "t", "text": "Fabeha Fazal, Lianzhi Gu, Ivanna Ihnatovych, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Inhibiting myosin light chain kinase induces apoptosis in vitro and in vivo."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biol (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/MCB.25.14.6259-6266.2005"}], "href": "https://doi.org/10.1128/MCB.25.14.6259-6266.2005"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15988034"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15988034"}]}, {"type": "r", "ref": 15, "children": [{"type": "t", "text": "Young Ok Choi, Hee Jeong Ryu, Hye Rim Kim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Implication of phosphorylation of the myosin II regulatory light chain in insulin-stimulated GLUT4 translocation in 3T3-F442A adipocytes."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Exp Mol Med (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/emm.2006.22"}], "href": "https://doi.org/10.1038/emm.2006.22"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16672772"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16672772"}]}, {"type": "r", "ref": 16, "children": [{"type": "t", "text": "Byung Joo Kim, Ju-Hong Jeon, Seon Jeong Kim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Can J Physiol Pharmacol (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1139/Y07-118"}], "href": "https://doi.org/10.1139/Y07-118"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18066127"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18066127"}]}, {"type": "r", "ref": 17, "children": [{"type": "t", "text": "Chongxiu Sun, Mack H Wu, Sarah Y Yuan "}, {"type": "b", "children": [{"type": "t", "text": "Nonmuscle myosin light-chain kinase deficiency attenuates atherosclerosis in apolipoprotein E-deficient mice via reduced endothelial barrier dysfunction and monocyte migration."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Circulation (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1161/CIRCULATIONAHA.110.988915"}], "href": "https://doi.org/10.1161/CIRCULATIONAHA.110.988915"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21670231"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21670231"}]}, {"type": "r", "ref": 18, "children": [{"type": "t", "text": "Ning Gao, Jian Huang, Weiqi He, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Signaling through myosin light chain kinase in smooth muscles."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M112.427112"}], "href": "https://doi.org/10.1074/jbc.M112.427112"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23362260"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23362260"}]}, {"type": "r", "ref": 19, "children": [{"type": "t", "text": "Shelly Woody, Richard Stall, Joseph Ramos, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Regulation of myosin light chain kinase during insulin-stimulated glucose uptake in 3T3-L1 adipocytes."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0077248"}], "href": "https://doi.org/10.1371/journal.pone.0077248"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24116218"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24116218"}]}, {"type": "r", "ref": 20, "children": [{"type": "t", "text": "Xia Yang, Jun-Guo Wang, Deng-Bin Ma, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Myosin light chain kinase regulates hearing in mice by influencing the F-actin cytoskeleton of outer hair cells and cochleae."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Mol Med (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/ijmm.2014.1634"}], "href": "https://doi.org/10.3892/ijmm.2014.1634"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24481113"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24481113"}]}, {"type": "r", "ref": 21, "children": [{"type": "t", "text": "Nobuko Serizawa, Jijing Tian, Hiroo Fukada, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Lab Invest (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/labinvest.2015.77"}], "href": "https://doi.org/10.1038/labinvest.2015.77"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26146960"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26146960"}]}, {"type": "r", "ref": 22, "children": [{"type": "t", "text": "William Gittings, Jordan Bunda, James T Stull, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Muscle Nerve (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/mus.25053"}], "href": "https://doi.org/10.1002/mus.25053"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26802366"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26802366"}]}, {"type": "r", "ref": 23, "children": [{"type": "t", "text": "Jie Han, Ji-Hong Li, Guang Bai, et al. "}, {"type": "b", "children": [{"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "Acanthopanax senticosus"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " polysaccharides-induced intestinal tight junction injury alleviation "}, {"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "via"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "World J Gastroenterol (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3748/wjg.v23.i12.2175"}], "href": "https://doi.org/10.3748/wjg.v23.i12.2175"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28405145"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28405145"}]}, {"type": "r", "ref": 24, "children": [{"type": "t", "text": "Kwang Hoon Song, Jaehyun Lee, Hong-Ryul Jung, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-017-11938-y"}], "href": "https://doi.org/10.1038/s41598-017-11938-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28912435"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28912435"}]}, {"type": "r", "ref": 25, "children": [{"type": "t", "text": "Jack L Williams, Anju Paudyal, Sherine Awad, et al. "}, {"type": "b", "children": [{"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "Mylk3"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " null C57BL/6N mice develop cardiomyopathy, whereas "}, {"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "Nnt"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " null C57BL/6J mice do not."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Life Sci Alliance (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.26508/lsa.201900593"}], "href": "https://doi.org/10.26508/lsa.201900593"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32213617"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32213617"}]}, {"type": "r", "ref": 26, "children": [{"type": "t", "text": "Toshio Kitazawa, Toshiyasu Matsui, Shuichi Katsuki, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A temporal Ca"}, {"type": "a", "children": [{"type": "t", "text": "sup"}], "href": "sup"}, {"type": "t", "text": "2+"}, {"type": "a", "children": [{"type": "t", "text": "/sup"}], "href": "/sup"}, {"type": "t", "text": " desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKβ/PP2A pathways."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Am J Physiol Cell Physiol (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1152/ajpcell.00136.2021"}], "href": "https://doi.org/10.1152/ajpcell.00136.2021"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34106787"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34106787"}]}]}]}