USP32 Gene

HGNC Family	Ubiquitin-specific peptidases (USP), EF-hand domain containing
Name	ubiquitin specific peptidase 32
Description	Enables cysteine-type deubiquitinase activity. Involved in positive regulation of TORC1 signaling. Located in cytosol. Is active in Golgi apparatus. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nUSP32 is an evolutionarily conserved deubiquitinating enzyme that plays key roles in regulating intracellular trafficking. It is localized to the Golgi and endosomal compartments, where it fine‐tunes protein sorting and maturation. Notably, USP32 deubiquitinates the small GTPase Rab7 to promote proper late endosome transport and recycling, and it counteracts the polyubiquitination of proprotein convertases such as furin, thereby regulating their exit from the trans‐Golgi network for subsequent post‐Golgi trafficking."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "USP32 also functions as an oncogenic driver in multiple cancer types. Elevated expression of USP32 has been associated with increased cellular proliferation, enhanced migration, and a greater propensity for epithelial–mesenchymal transition, correlating with poor clinical outcomes. Its overexpression or amplification has been observed in malignancies such as breast cancer, small cell lung cancer, gastric cancer, ovarian cancer, glioblastoma, and hepatocellular carcinoma, while its suppression results in reduced SMAD2 levels and impaired tumor progression."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "4", "end_ref": "8"}]}, {"type": "t", "text": "Moreover, USP32 modulates the stability of key substrates that determine drug responses and resistance. It governs the protein levels of factors such as SLC35F2—affecting the cellular uptake of the anticancer compound YM155—as well as Rab35, whose stabilization underlies exosome‐mediated transmission of imatinib resistance in gastrointestinal stromal tumors. In acute myeloid leukemia, USP32 deubiquitinates Rap1b, thereby promoting oncogenic signaling, and its expression appears to be influenced by a regulatory network involving circular RNA and microRNAs."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "9", "end_ref": "11"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Aysegul Sapmaz, Ilana Berlin, Erik Bos, et al. "}, {"type": "b", "children": [{"type": "t", "text": "USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-019-09437-x"}], "href": "https://doi.org/10.1038/s41467-019-09437-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30926795"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30926795"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Shiva Akhavantabasi, Hesna B Akman, Aysegul Sapmaz, et al. "}, {"type": "b", "children": [{"type": "t", "text": "USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mamm Genome (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s00335-010-9268-4"}], "href": "https://doi.org/10.1007/s00335-010-9268-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20549504"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20549504"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Wenqiang Su, Iqbal Ahmad, You Wu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Adv Sci (Weinh) (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/advs.202403732"}], "href": "https://doi.org/10.1002/advs.202403732"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "39031635"}], "href": "https://pubmed.ncbi.nlm.nih.gov/39031635"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Wenyu Hu, Haiyan Wei, Keming Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Downregulation of USP32 inhibits cell proliferation, migration and invasion in human small cell lung cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Prolif (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/cpr.12343"}], "href": "https://doi.org/10.1111/cpr.12343"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28597490"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28597490"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Ning Dou, Qingqing Hu, Li Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "USP32 promotes tumorigenesis and chemoresistance in gastric carcinoma via upregulation of SMAD2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Biol Sci (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7150/ijbs.43117"}], "href": "https://doi.org/10.7150/ijbs.43117"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32226309"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32226309"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Aya Nakae, Michiko Kodama, Toru Okamoto, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Ubiquitin specific peptidase 32 acts as an oncogene in epithelial ovarian cancer by deubiquitylating farnesyl-diphosphate farnesyltransferase 1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2021.03.049"}], "href": "https://doi.org/10.1016/j.bbrc.2021.03.049"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33744759"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33744759"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Sifang Chen, Xi Chen, Zhangyu Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of ubiquitin-specific protease 32 as an oncogene in glioblastoma and the underlying mechanisms."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-022-09497-y"}], "href": "https://doi.org/10.1038/s41598-022-09497-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35440702"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35440702"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Mengxi Xiu, Wenfang Bao, Jialin Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "High USP32 expression contributes to cancer progression and is correlated with immune infiltrates in hepatocellular carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Cancer (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12885-023-11617-4"}], "href": "https://doi.org/10.1186/s12885-023-11617-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37957631"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37957631"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Arun Pandian Chandrasekaran, Kamini Kaushal, Chang-Hwan Park, et al. "}, {"type": "b", "children": [{"type": "t", "text": "USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Theranostics (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7150/thno.63806"}], "href": "https://doi.org/10.7150/thno.63806"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34815782"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34815782"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Chao Li, Zhishuang Gao, Zhiwei Cui, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Deubiquitylation of Rab35 by USP32 promotes the transmission of imatinib resistance by enhancing exosome secretion in gastrointestinal stromal tumours."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41388-023-02600-1"}], "href": "https://doi.org/10.1038/s41388-023-02600-1"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36725886"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36725886"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Heyang Zhang, Yuan Tao, Xin Ding, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Acta Biochim Biophys Sin (Shanghai) (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3724/abbs.2023037"}], "href": "https://doi.org/10.3724/abbs.2023037"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36951484"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36951484"}]}]}]}
Synonyms	NY-REN-60, USP10
Proteins	UBP32_HUMAN
NCBI Gene ID	84669
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

USP32 has 7,993 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 112 datasets.

Click the + buttons to view associations for USP32 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

USP32 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by USP32 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of USP32 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of USP32 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of USP32 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of USP32 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of USP32 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of USP32 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of USP32 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of USP32 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of USP32 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with USP32 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with USP32 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of USP32 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of USP32 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of USP32 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of USP32 gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing USP32 protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of USP32 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing USP32 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing USP32 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing USP32 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with USP32 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of USP32 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with USP32 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with USP32 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with USP32 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by USP32 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with USP32 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with USP32 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with USP32 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with USP32 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at USP32 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of USP32 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of USP32 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing USP32 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with USP32 gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GAD High Level Gene-Disease Associations	diseases associated with USP32 gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of USP32 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with USP32 gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing USP32 from the GeneSigDB Published Gene Signatures dataset.