GSE69750 | Datasets2Tools

General Information

Accession	GSE69750
Title	Gene expression profiling of six MEF cell genotypes (wild type, 2SP+/-, 2SP-/-, SMAD3+/-, SMAD3-/-, and 2SP+/-; SMAD3+/-)
Description	Gene expression profiling was carried out in six (wild type, 2SP+/-, 2SP-/-, SMAD3+/-, SMAD3-/- and 2SP+/-/ SMAD3+/-) different mouse knockout embryonic fibroblast (MEF) cells. Beta-2-spectrin (2SP) is a dynamic intracellular non-pleckstrin homology (PH)-domain protein that belongs to a family of polypeptides that have been implicated in conferring cell polarity. Spectrins have been linked to multiple signaling pathways, including cell cycle regulation, DNA repair and TGF signaling. In this study, we report a major role of the TGF/Smad3 adaptor 2-Spectrin in conserving genomic integrity from alcohol-induced DNA damage and describe a novel pathway that protects genomes from genotoxic stresses. To determine the mechanism for the oncogenic switch, and whether it is related to the role of 2SP in TGF- signaling transduction or secondary to its cytoskeletal functions, we analyzed disruption of two elements of the TGF- pathway by generating double heterozygous Sptbn1+//Smad3+/ mice.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE69750
Repository	Gene Expression Omnibus