A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation
Transcriptomics Dataset
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General Information
AccessionGSE72105
TitleA specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation
DescriptionTo study the function of E3 ligase Huwe1 knockdown on C2C12 proliferation and differentiation, we use mRNA-seq to compare gene expression profiles between control wild-type C2C12 myoblasts and two Huwe1 knockdown C2C12 cell lines generated by different shRNAs on differentiation Day 0 (proliferation medium) and Day 6 (differentiation medium for 6 days). To study the potential roles of Huwe1 in maintainance of muscle morphology, we use mRNA-seq to compare gene expression profiles between control and Huwe1 knockdown myotubes. To study whether downregulation of USP10 deubiquitinase is of functional significance during muscle differentiation, we compare gene expression profiles between control wildtype and USP10 overexpressing C2C12 cells on differentiation Day 6.
Landing Pagehttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72105
RepositoryGene Expression Omnibus
Tools used to analyze the dataset
Dataset Accession
ARCHS4
Published September 2017
ARCHS4 provides access to gene counts from HiSeq 2000 and HiSeq 2500 platforms for human and mouse experiments from GEO ...
4645
Clustergrammer
Published October 2017
Web-based tool for visualizing and analyzing high-dimensional data as interactive and shareable heatmaps
4645
Canned Analyses generated with the dataset
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Tool Name
Interactive heatmap visualization of RNA-seq dataset GSE72105
ARCHS4, Clustergrammer
Highly interactive web-based heatmap visualization of the top 500 most variable genes in t...
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