GSE76970 | Datasets2Tools

General Information

Accession	GSE76970
Title	Reversion to nave human pluripotency creates a new methylation landscape devoid of blastocyst or germline memory
Description	Human embryonic stem cells (hESCs) typically exhibit 'primed' pluripotency, analogous to stem cells derived from the mouse post-implantation epiblast. This has led to a search for growth conditions that support self-renewal of hESCs akin to hypomethylated nave epiblast cells in human pre-implantation embryos. We have discovered that reverting primed hESCs to a hypomethylated nave state or deriving a new hESC line under nave conditions results in the establishment of Stage Specific Embryonic Antigen 4 (SSEA4) negative hESC lines with a transcriptional program resembling the human pre-implantation epiblast. In contrast, we discovered that the methylome of nave hESCs in vitro is distinct from the human epiblast in vivo with loss of DNA methylation at primary imprints and a lost 'memory' of the methylation state of the human oocyte. This failure to recover the nave epiblast methylation landscape appears to be a consistent feature of self-renewing hypomethylated nave hESCs in vitro.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE76970
Repository	Gene Expression Omnibus