General Information
| Description | Prediction of effects of amino acid substitutions on protein solubility |
| Homepage | http://structure.bmc.lu.se/PON-Sol |
Publications
PON-Sol: prediction of effects of amino acid substitutions on protein solubility
Motivation:
Solubility is one of the fundamental protein properties. It is of great interest because of its relevance to protein expression. Reduced solubility and protein aggregation are also associated with many diseases.
Results:
We collected from literature the largest experimentally verified solubility affecting amino acid substitution (AAS) dataset and used it to train a predictor called PON-Sol. The predictor can distinguish both solubility decreasing and increasing variants from those not affecting solubility. PON-Sol has normalized correct prediction ratio of 0.491 on cross-validation and 0.432 for independent test set. The performance of the method was compared both to solubility and aggregation predictors and found to be superior. PON-Sol can be used for the prediction of effects of disease-related substitutions, effects on heterologous recombinant protein expression and enhanced crystallizability. One application is to investigate effects of all possible AASs in a protein to aid protein engineering.
Availability and implementation:
PON-Sol is freely available at http://structure.bmc.lu.se/PON-Sol. The training and test data are available at http://structure.bmc.lu.se/VariBench/ponsol.php
Contact:
mauno.vihinen@med.lu.se
Supplementary information:
Supplementary data are available at Bioinformatics online.
Related Tools
cc SOL omics
Published June 2014
A webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli
PROVEAN web server
Published April 2015
A tool to predict the functional effect of amino acid substitutions and indels
INPS
Published May 2015
Predicting the impact of non-synonymous variations on protein stability from sequence
PreDisorder
Published December 2009
Ab initio sequence-based prediction of protein disordered regions
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
