General Information
| Description | A webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli |
| Homepage | http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html |
Publications
cc SOL omics : a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli
Summary:
Here we introduce cc SOL omics , a webserver for large-scale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis.
Results:
Using coil/disorder, hydrophobicity, hydrophilicity, β-sheet and α-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing <30% sequence similarity.
Availability and implementation:
cc SOL omics can be freely accessed on the web at http://s.tartaglialab.com/page/ccsol_group . Documentation and tutorial are available at http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html .
Contact:
gian.tartaglia@crg.es
Supplementary information:
Supplementary data are available at Bioinformatics online.
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