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SOFT file
| Title / Description |
6KR-EGFR expressing MCF7 following EGF or HRG stimulation |
| Accession |
GSE57547
|
| Summary |
EGF and HRG, growth factor ligands for EGFR and ErbB3/4 receptor, induce transient and sustained ERK activity associated with cellular proliferation and differentiation of MCF-7 cells, respectively. To rigorously analyze the effect of ERK signal duration for mRNA expression dynamics and its relationship with cell determination, we modified the EGF-triggered ERK signal duration by changing the EGFR activation dynamics by impairing the ubiquitination and degradation process. Mutation of the six lysine residues (6KR; K692, K713, K730, K843, K905 and K946) of the EGFR responsible for ubiquitin conjugation has shown sustained phosphorylation of the receptor (Huang et al, 2006; Goh et al, 2010). Therefore we constructed the MCF-7 cell lines that stably express 6KR EGFR (6KR), and analyzed signaling and mRNA expression dynamics in response to EGF and HRG. |
| Organism |
Homo sapiens |
| Platform |
GPL570 |
| Normalized |
False |
| Parsed SOFT file |
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Metadata
| Differential expression method |
Characteristic Direction
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| Cutoff |
500 |
| Organism |
Homo sapiens |
| Cell_type |
MCF7cells |
| Pert_name |
heregulin |
| Pert_id |
HRG |
| Organism |
human |
| Pert_type |
chemical |
Visualizations
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External Links and Downloads
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Gene lists
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Enrichr
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L1000CDS2
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PAEA
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