Effect of Brwd1 mutation on the mouse oocyte transcriptome
Transcriptomics Dataset
FAIR Metrics
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General Information
AccessionGSE56724
TitleEffect of Brwd1 mutation on the mouse oocyte transcriptome
DescriptionThe Brwd1 mutation, though having dramatic effects on the spermatocyte transcriptome, had little effect on the oocyte transcriptome. Postmeiotic gene expression is essential for development and maturation of sperm and eggs. In B6/C3H females, Brwd1 ablation causes severe chromosome condensation and structural defects associated with abnormal telomere structure. In contrast to males, transcriptome analysis at the germinal vesicle stage revealed only minor changes in gene expression including a >2 fold overexpression of the histone methyltransferase MLL5 and LINE-1 elements transposons. Thus, loss of BRWD1 function interferes with the completion of oogenesis and spermatogenesis through sexually dimorphic mechanisms. Our results demonstrate that BRWD1 is essential for the epigenetic control of chromosome stability during female meiosis while playing a critical role in haploid gene transcription during the post-meiotic differentiation events of spermiogenesis.
Landing Pagehttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE56724
RepositoryGene Expression Omnibus
Tools used to analyze the dataset
Keyword
Dataset Accession
Enrichr
Published May 2016
A comprehensive gene set enrichment analysis web server 2016 update
7759
65
Characteristic Direction
Published March 2014
A geometrical multivariate approach to identify differentially expressed genes
26829
GeneMANIA
Published June 2010
Biological network integration for gene prioritization and predicting gene function
7435
L1000CDS2
Published August 2016
An ultra-fast LINCS L1000 Characteristic Direction signature search engine
7756
PAEA
Published November 2015
Enrichment analysis tool implementing the principal angle method
3879
Canned Analyses generated with the dataset
Filters  
Dataset Accession
Tool Name
Direction
Geneset
Small molecules which mimic BRWD1 Mutation
L1000CDS2, Characteristic Direction
The L1000 database was queried in order to identify small molecule perturbations which mim...
Small molecules which reverse BRWD1 Mutation
L1000CDS2, Characteristic Direction
The L1000 database was queried in order to identify small molecule perturbations which rev...
Enrichment analysis of genes dysregulated in BRWD1 Mutation
PAEA, Characteristic Direction
An enrichment analysis was performed on the top most dyresgulated genes determined by appl...
Interaction network and enrichment analysis of genes downregulated in BRWD1 Mutation
GeneMANIA, Characteristic Direction
The analysis explores the gene interaction network and pathway enrichment of the top 50 mo...
Interaction network and enrichment analysis of genes upregulated in BRWD1 Mutation
GeneMANIA, Characteristic Direction
The analysis explores the gene interaction network and pathway enrichment of the top 50 mo...
Enrichment analysis of genes downregulated in BRWD1 Mutation
Enrichr, Characteristic Direction
An enrichment analysis was performed on the top 500 most downregulated genes identified by...
Enrichment analysis of genes upregulated in BRWD1 Mutation
Enrichr, Characteristic Direction
An enrichment analysis was performed on the top 500 most upregulated genes identified by a...
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