Expression data from U-87 human glioma cells with proficient and deficient NF-B after treatment with temozolomide
Transcriptomics Dataset
FAIR Metrics
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General Information
AccessionGSE65363
TitleExpression data from U-87 human glioma cells with proficient and deficient NF-B after treatment with temozolomide
DescriptionThe transcription factor, NF-B, plays a central role in the response to DNA damage. This ubiquitous family of proteins is made up of five subunits: p50 (NF-B1, p105), p52 (NF-B2, p100), p65 (relA), relB, and crel that appear in their mature form as dimers. Following stimulation, NF-B dimers translocate to the nucleus where they bind specific consensus elements (B-sites) in the promoter region of genes involved in cell survival, inflammation and the immune system. While there is a general propensity of NF-B to mediate survival, this is not always the case and several reports note the pro-apoptotic nature of the NF-B pathway. In examining the NF-B response to DNA damage, we have found that the p50 subunit plays a central role in modulating cytotoxicity following TMZ treatment in malignant glioma. In the current study, given the importance of p50 to the cytotoxic response to TMZ, we set out to identify NF-B-dependent factors that modulate the response to TMZ.
Landing Pagehttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65363
RepositoryGene Expression Omnibus
Tools used to analyze the dataset
Keyword
Dataset Accession
Enrichr
Published May 2016
A comprehensive gene set enrichment analysis web server 2016 update
7759
65
Characteristic Direction
Published March 2014
A geometrical multivariate approach to identify differentially expressed genes
26829
GeneMANIA
Published June 2010
Biological network integration for gene prioritization and predicting gene function
7435
L1000CDS2
Published August 2016
An ultra-fast LINCS L1000 Characteristic Direction signature search engine
7756
PAEA
Published November 2015
Enrichment analysis tool implementing the principal angle method
3879
Canned Analyses generated with the dataset
Filters  
Dataset Accession
Tool Name
Direction
Geneset
Small molecules which mimic NFKB1 KD
L1000CDS2, Characteristic Direction
The L1000 database was queried in order to identify small molecule perturbations which mim...
Small molecules which reverse NFKB1 KD
L1000CDS2, Characteristic Direction
The L1000 database was queried in order to identify small molecule perturbations which rev...
Enrichment analysis of genes dysregulated in NFKB1 KD
PAEA, Characteristic Direction
An enrichment analysis was performed on the top most dyresgulated genes determined by appl...
Interaction network and enrichment analysis of genes downregulated in NFKB1 KD
GeneMANIA, Characteristic Direction
The analysis explores the gene interaction network and pathway enrichment of the top 50 mo...
Interaction network and enrichment analysis of genes upregulated in NFKB1 KD
GeneMANIA, Characteristic Direction
The analysis explores the gene interaction network and pathway enrichment of the top 50 mo...
Enrichment analysis of genes downregulated in NFKB1 KD
Enrichr, Characteristic Direction
An enrichment analysis was performed on the top 500 most downregulated genes identified by...
Enrichment analysis of genes upregulated in NFKB1 KD
Enrichr, Characteristic Direction
An enrichment analysis was performed on the top 500 most upregulated genes identified by a...
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